The Store Mosse National Park in the south of Sweden was surveyed for nematode diversity and distribution using DNA metabarcoding. Fifty samples were collected from five vegetation types in the park across a range of habitats (e.g. soil, litter, lichens, sphagnum and roots). The other habitats, aside from soil and litter, were sampled in order to capture the diversity of nematodes that may be uniquely associated with them. Nematodes were characterised using the V7-V8 variable domain (~ 350 bp) of the 18S ribosomal RNA gene. We identified 46 families, 76 genera (21 new to Swedish fauna) and 60 species (31 new to Swedish fauna). Some nematodes showed strong associations with their habitats, especially at the species level. Although soil and litter supported the most diverse nematode communities, our results support a strong justification for sampling across different media types to quantify nematode diversity accurately. Soil and litter communities showed high levels of stability with balanced distribution of all the various trophic and coloniser-persister groups.