TRIBE LAB @ INESC TEC datasets
Published January 12, 2024 | Version v1
Dataset Restricted

Hyperspectral spectroscopic reflectance data collected in-vivo non-symptomatic and symptomatic kiwi leaves in field conditions

Creators

  • 1. ROR icon Institute for Systems Engineering and Computers
  • 2. ROR icon INESC TEC
  • 3. Faculdade de Ciências da Universidade do Porto (FCUP)
  • 4. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado
  • 5. BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO

Description

Hyperspectral spectroscopic reflectance data collected in-vivo non-symptomatic and symptomatic kiwi leaves in field conditions

Study Area

The monitoring of kiwi plants (Actinidia deliciosa) was performed in two test sites, integrated in commercial orchards at Guimarães, Portugal, located in Caldas das Taipas (CT; 41°29′09.8′′ N 8°21′54.3′′ W) and Briteiros (BT; 41°30′53.3′′ N 8°19′20.5′′ W). In CT, the orchard was 5 years old (2020), and 12 female kiwi plants of the variety Bo.Erika®were assessed. In the BT test site, plants had 30 years old (2020), and 8 feminine plants of the same variety were selected to integrate the study.

Visual disease identification (scouting) was accomplished by the detection of characteristic symptoms of bacterial kiwi canker, caused by Pseudomonas syringae pv. actinidiae on leaves (adaxial and abaxial sides). Samples were classified as asymptomatic (showing no symptoms) or symptomatic (presenting at least one typical chlorotic or necrotic spot).

Spectral Ground Measurements

Leaf hyperspectral data were obtained with a portable spectroradiometer (ASD FieldSpec® HandHeld 2, ASD Instruments, Boulder, CO, USA). Reflectance data were recorded in the wavelength range from 325 nm to 1075 nm, with 1 nm of spectral resolution. The spectroradiometer has a full conical field-of-view angle of 25°. During the data acquisition, the sensor was maintained 30 cm above the kiwi leaf, directed vertically downward (nadir view), giving a sampling footprint close to 13.3 cm. The leaf was placed upon a black card to reduce background noise. Prior to the hyperspectral acquisition, an internal dark calibration was performed, followed by a white calibration through a spectralon (white reference panel).
Measurements were acquired in the nadir position, in cloud-free conditions, between 11:00 and 14:00 h (local time), minimizing changes in the solar zenith angle. Weekly hyperspectral data on plant’s reflectance were obtained between May and June 2020, which corresponded to the full development of Psa symptoms in kiwi plant leaves during the growing season. After, biweekly measurements were performed between July and August 2020. Three random leaves were chosen for each plant, and hyperspectral information was collected from one point, totaling 504 measurement points. In each spectral measurement, 10 repetitions were performed and later averaged to minimize the noise effect.

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Additional details

Related works

Is described by
Publication: 10.3390/plants11162154 (DOI)

Funding

European Union
European Union’s Horizon 2020 Grant Agreement 857202
Fundação para a Ciência e Tecnologia
Doctoral Grant SFRH/BD/146564/2019]
European Commission
Project Grant 101004085