Published January 12, 2024 | Version v1
Dataset Restricted

Hyperspectral spectroscopic transmittance data collected in-vivo healthy and diseased tomato leaflets in controlled conditions - dataset II

  • 1. ROR icon Institute for Systems Engineering and Computers
  • 2. ROR icon Institute for Systems and Computer Engineering of Porto
  • 3. Faculdade de Ciências da Universidade do Porto (FCUP)
  • 4. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado
  • 5. BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO

Description

Hyperspectral Spectroscopic Transmittance data collected in-vivo tomato leaflets in controlled conditions

Hyperspectral point-of-measurements (HS-POM) were collected in vivo from the adaxial side of healthy and diseased leaflets of nine tomato plants, in a dark room (in detail, three Control plants, three plants inoculated with Pseudomonas syringae pv. tomato, and three plants inoculated with Xanthomonas euvesicatoria). For each plant, spectral assessments were performed randomly on nine points of different leaflets, belonging to the 4th, 5th, and 6th expanded leaves.

Hyperspectral data were acquired using a Hamamatsu Photonics K.K. TM Series C11697MB spectrometer, which covers a wavelength range of 200-1100 nm with a spectral resolution of 0.6 nm. A transmission optical fiber bundle (FCR-7UVIR200-2-45-BX, Avantes, Eerbeek, The Netherlands) with a range of 200-2500 nm was used along with a stainless-steel slitted reflection probe that was positioned 0.5 cm above the sample surface to capture the leaflet's spectral signal and direct it to the spectrometer's entrance lens. A white LED light was placed underneath the leaflet to provide uniform illumination to its entire abaxial surface. The spectral range of the LED emits light from 390 to 800 nm. Therefore, the LED spectra were used as a reference to the spectral range measured by the spectrophotometer and to check measurement and light emission stability. The hyperspectral transmittance data were collected using specialized evaluation software (SpecEvaluationUSB2.exe, Hamamatsu Photonics K.K., Japan).

For more detailed description of the bacterial inoculation protocol please check:

Reis Pereira M, Santos FNd, Tavares F and Cunha M (2023) Enhancing host-pathogen phenotyping dynamics: early detection of tomato bacterial diseases using hyperspectral point measurement and predictive modeling. Front. Plant Sci. 14:1242201. doi: 10.3389/fpls.2023.1242201

Files

Restricted

The record is publicly accessible, but files are restricted to users with access.

Additional details

Funding

Doctoral Grant SFRH/BD/146564/2019]
Fundação para a Ciência e Tecnologia
Project Grant PTDC/ASP-HOR/1338/2021
Fundação para a Ciência e Tecnologia
Project Grant 101004085
European Commission