Published August 20, 2015
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Fig. 5 in Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPHcytochrome P450 reductase CPR I from Croton stellatopilosus Ohba
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- 1. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand & Research Unit for Natural Product Biotechnology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Description
Fig. 5. Heterologous expression of the recombinant CsCPR I (A) and CYP76F45 (B) proteins in E. coli analyzed by 12% SDS–PAGE. (A) Lane 1, empty pET32a in E. coli; lanes 2–7 show protein patterns of cell lysates obtained after inducing with IPTG for 1, 2, 4, 6, 18 and 40 h, respectively. (B) Lane 1, empty pET32a in E. coli; lanes 2–6 show protein patterns of cell lysates obtained after inducing with IPTG for 1, 2, 6, 18 and 40 h, respectively; lane 7 shows the pattern of un-induced cell lysate for 40 h. Lane M shows the molecular markers. (C) Immunoblot with alkaline phosphatase (AP)-conjugated antibodies for the 6His-tag at the N-terminal of CsCPR I and the C-terminal of CYP76F45.
Notes
Published as part of Sintupachee, Siriluk, Promden, Worrawat, Ngamrojanavanich, Nattaya, Sitthithaworn, Worapan & De-Eknamkul, Wanchai, 2015, Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPHcytochrome P450 reductase CPR I from Croton stellatopilosus Ohba, pp. 204-215 in Phytochemistry 118 on page 210, DOI: 10.1016/j.phytochem.2015.08.005, http://zenodo.org/record/10488806
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- Journal article: 10.1016/j.phytochem.2015.08.005 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFDEFF9AFF8E233CF65BFFE1FFB2FFC4 (LSID)
- Journal article: https://zenodo.org/record/10488806 (URL)