Fig. 2. Amino acid sequence alignment and structure predictions for UGT72E, UGT72E3, and their recombinants. (A) Amino acid sequence alignment and secondary structure prediction for UGT72E2 and UGT72E3. Sequence identity (85.7%) is shaded dark gray, and similarity (91.1%) is shaded light gray. The diagnostic PSPG motif of plant family 1 glucosyltransferases is underlined. The exchange site for recombinant UGT72E2/3 and UGT72E3/2 is indicated with an arrowhead. The secondary structures of UGT72E2 and UGT72E3 were predicted using the SWISS-MODEL workspace. The structures are almost identical, but differences are marked with boxes. A β-strand and an oi-helix structure are illustrated by an arrow and a wavy line, respectively. (B) The 3D structures of UGT72E2, UGT72E3 and their recombinant UGTs, UGT72E2/3 and UGT72E3/2, were predicted with the SWISS-MODEL workspace using UGT72B1as a template. Each protein was diagrammed with color-ramped way from the N (blue) to the C terminus (red). Regions where UGT72E2 and UGT72E3 display different secondary structures are indicated.