Fig. 2 in Ryecyanatines A and B and ryecarbonitrilines A and B, substituted cyanatophenol, cyanatobenzo[1,3]dioxole, and benzo[1,3]dioxolecarbonitriles from rye (Secale cereale L.) root exudates: Novel metabolites with allelopathic activity on Orobanche seed

Fig. 2. (A) Seed germination of Orobanche crenata (a), O. cumana (b) and O. minor (c) after 7 days of stimulation with ryecarbonitriline A (3), GR24 (positive control) or negative control (distilled water+methanol). No germination was observed in Orobanche seeds treated with ryecarbonitriline B, ryecyanatine A and B (data not shown). Each bar represents the mean of three biological replicates±SE. (B) Inhibition mediated by ryecyanatine A (1) of seed germination of Orobanche crenata (a), O. cumana (b) and O. minor (c) at a concentration range of 0.60–0.06 mM. Each bar represents the mean of three biological replicates±SE. (C) Inhibition mediated by ryecarbonitriline B (4) of seed germination of Orobanche crenata (a), O. cumana (b) and O. minor (c) at a concentration range of 0.70–0.07 mM. Each bar represents the mean of three biological replicates±SE.