Published July 9, 2015
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Fig. 4 in Sequential oxidation of Jasmonoyl-Phenylalanine and JasmonoylIsoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates
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- 1. Département Réseaux Métaboliques chez les Végétaux. Institut de Biologie Moléculaire des Plantes, UPR 2357 du Centre National de la Recherche Scientifique,
Description
Fig. 4. UPLC-MS/MS analysis of metabolites produced by CYP94B1 and CYP94B3 with JA-Phe. JA-Phe was incubated with microsomes of yeast expressing CYP94B1 in presence (panels A–D) or in absence (panels E–H) of NADPH and with microsomes of yeast expressing CYP94B3 in presence (panels I-L) or in absence (panels M-P) of NADPH. JA-Phe (100 μM) and microsomes (0.4 mg protein) were incubated for 20 min at 27 °C. Residual substrate (JA-Phe) and metabolites (12OH-JA-Phe, 12CHO-JA-Phe and 12COOH-JA-Phe) were identified by LC-MS/MS based on their retention times and detection in multiple reaction monitoring.
Notes
Published as part of Widemann, Emilie, Grausem, Bernard, Renault, Hugues, Pineau, Emmanuelle, Heinrich, Clément, Lugan, Raphaël, Ullmann, Pascaline, Miesch, Laurence, Aubert, Yann, Miesch, Michel, Heitz, Thierry & Pinot, Franck, 2015, Sequential oxidation of Jasmonoyl-Phenylalanine and JasmonoylIsoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates, pp. 388-399 in Phytochemistry 117 on page 392, DOI: 10.1016/j.phytochem.2015.06.027, http://zenodo.org/record/10486154
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- Journal article: 10.1016/j.phytochem.2015.06.027 (DOI)
- Journal article: urn:lsid:plazi.org:pub:443B2A46854FFF90D239FFECFC5B704A (LSID)
- Journal article: https://zenodo.org/record/10486154 (URL)