Published September 30, 2015
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Fig. 1 in Discovery of small molecule inhibitors of xyloglucan endotransglucosylase (XET) activity by high-throughput screening
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- 1. The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, The King's Buildings, Max Born Crescent, Edinburgh EH9 3BF, UK
Description
Fig. 1. Dot-blot screening for XET activity in total extracts from diverse plant organs. (a) XET assays in 96-well format, on paper impregnated with 0.3% xyloglucan + 5 μM XGO–SR; (b) control assays on paper impregnated with XGO–SR alone. Rows A–D and E–H show results with low- and high-salt extracts, respectively, from the plant organs listed on the right. The enzyme solutions (4 μl) were incubated on the papers for 13 h at 22 °C. (c) XET assays on paper impregnated with 0.3% xyloglucan + 5 μM XGO–SR. The enzyme extracts (low-salt buffer) were from parsley (P) or asparagus (A), and either undiluted (row '1') or 2–8-fold diluted (rows '/2' to '/8'); 4 μl was applied to the paper and incubated at 22 °C for 0.5, 1, 2, 4, 6 or 12 h, as indicated.
Notes
Published as part of Chormova, Dimitra, Franková, Lenka, Defries, Andrew, Cutler, Sean R. & Fry, Stephen C., 2015, Discovery of small molecule inhibitors of xyloglucan endotransglucosylase (XET) activity by high-throughput screening, pp. 220-236 in Phytochemistry 117 on page 223, DOI: 10.1016/j.phytochem.2015.06.016, http://zenodo.org/record/10486003
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- Journal article: 10.1016/j.phytochem.2015.06.016 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFD9FFF7FFE2FFBFE367FF8EFFFE3A2D (LSID)
- Journal article: https://zenodo.org/record/10486003 (URL)