Published February 28, 2015 | Version v1
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Fig. 7 in Effects of synthetic alkamides on Arabidopsis fatty acid amide hydrolase activity and plant development

  • 1. Center for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA

Description

Fig. 7. Amidohydrolase activity (µmol min–1 mg of protein–1) of purified FAAH protein with different [1-14C]N-acylethanolamines (NAEs) in presence of 100 µM of N-isopropyllauroylamide (3) or N-ethyllauroylamide (4). Reactions were initiated by the addition of purified FAAH (0.3 µg) in 50 mM Bis-Tris propane–HCl (pH 9.0) and different radiolabeled NAEs (12:0, 16:0 and 20:4) in a final volume of 0.15 ml. Reactions proceeded at 30 °C, 30 min, with shaking (120 rpm). Data points represent means ± S.D. of triplicate assays. Plots were generated with SigmaPlot software version 12.0. p-Value of <0.05 is indicated by * as determined by Student's t test. ns, not significant.

Notes

Published as part of Faure, Lionel, Cavazos, Ronaldo, Khan, Bibi Rafeiza, Petros, Robby A., Koulen, Peter, Blancaflor, Elison B. & Chapman, Kent D., 2015, Effects of synthetic alkamides on Arabidopsis fatty acid amide hydrolase activity and plant development, pp. 58-71 in Phytochemistry 110 on page 63, DOI: 10.1016/j.phytochem.2014.11.011, http://zenodo.org/record/10485900

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Journal article: 10.1016/j.phytochem.2014.11.011 (DOI)
Journal article: urn:lsid:plazi.org:pub:FFD2DA38DE0D871A8D59FFF9FFA3FFE7 (LSID)
Journal article: https://zenodo.org/record/10485900 (URL)