Published December 31, 2018
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Fig. 4. C-22 in A cytochrome P450 monooxygenase responsible for the C-22 hydroxylation step in the Paris polyphylla steroidal saponin biosynthesis pathway
Authors/Creators
- 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, PR China & School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, PR China
- 2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, PR China & State Key Laboratory of Breeding Base Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700,
- 3. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, PR China
Description
Fig. 4. C-22 Hydroxylation of cholesterol by PpCYP90B27 in yeast. GC-MS analysis of the fermentation products from the recombinant yeast expressing PpCYP90B27 in MRM mode. The precursor ions with m/z of 329.1 were bombarded with 15 eV collisional energy from the 8th minute, and product ions with m/z of 121.1 and 95.1 were scanned. Precursor ions with an m/z of 173.0 were bombarded with 5 eV collisional energy and product ions with m/z of 83.1 and 73.0 were scanned starting at 10.7 min.
Notes
Published as part of Yin, Yan, Gao, Linhui, Zhang, Xianan & Gao, Wei, 2018, A cytochrome P450 monooxygenase responsible for the C-22 hydroxylation step in the Paris polyphylla steroidal saponin biosynthesis pathway, pp. 116-123 in Phytochemistry 156 on page 119, DOI: 10.1016/j.phytochem.2018.09.005, http://zenodo.org/record/10484444
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- Journal article: 10.1016/j.phytochem.2018.09.005 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFE16558B062FFBC8E48FFA19F6CF63B (LSID)
- Journal article: http://publication.plazi.org/id/FFE16558B062FFBC8E48FFA19F6CF63B (URL)
- Journal article: https://zenodo.org/record/10484444 (URL)