Fig. 2. Transcriptional analysis of MnPPO genes from M. notabilis. (a) Transcriptional analysis of MnPPO genes in five tissues (root, bark, bud, flower, and leaf) of M. notabilis. (b) Transcriptional analysis of MnPPO genes in leaf following UV exposure. (c) Transcriptional analysis of MnPPO genes in leaf after drought treatment. (d) Transcriptional analysis of MnPPO genes in leaf after gray mold treatment. (e) Immunoblotting analysis of MnPPO1 in (d). (f) Immunoblotting analysis of the antibody specificity of MnPPO1. MnPPO1 antigen and rabbit lgG were used as positive and negative control, respectively. M. notabilis seedlings grown under the same conditions were exposed to UV-C for 0, 20, 40, 60, or 80 min or underwent drought treatment for 0, 2, 4, 6 or 8 d or gray mold treatment for 0, 8, 24, 48, 72 or 92 h. Leaves of treated seedlings were harvested directly into liquid nitrogen and stored at −80 ̊C for total RNA extraction. Corresponding tissues of untreated seedlings were used as controls. Relative expression of genes was obtained by qPCR. Values are shown as the means ± SD (n = 3).