Published March 18, 2019 | Version v1
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Fig. 1 in In vitro evaluation of hydroxycinnamoyl CoA:quinate hydroxycinnamoyl transferase expression and regulation in Taraxacum antungense in relation to 5-caffeoylquinic acid production

  • 1. ∗ & College of Horticulture, Shenyang Agricultural University, Shen Yang, 110866, China & ∗ & Exsitu Conservation Garden Evaluation Centre of Wild Vegetable Germplasm in Northeast China under Ministry of Agriculture, Shen Yang, 110866, China

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Fig. 1. Multiple alignment, phylogenetic analysis, and organ expression analysis of TaHQT1 and TaHQT2. a. Sequence alignment of HQTs sequences in T.antungese. Multiple sequence alignment of TaHQT1 (in this article, MK033609), TaHQT2 (MH683053), CcHQT1 (ADL62854.1), CcHQT2 (ACJ23164.1), NtHQT (CAE46932.1), and CiHQT (ANN12610.1) The conserved domain PLN02663 (HXXXD) and pfam02458 (DFGWG) is indicated by a straight line. Black boxes indicate the conserved structural motifs in the acyl-transferase family. b. Phylogenetic analysis of hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase. The phylogenetic tree was constructed using MEGA software (version7.0) based on the neighbour-joining method. Values above the branches are bootstrap percentages (1000 replicates). Abbreviations and gene ID were as follows. TaHQT1 (this article, MK033609), Taraxacum antungense hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase 1; TaHQT2 (MH683053), Taraxacum antungense hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase 2; CiHQT(ANN12610.1) Cichorium intybus hydroxycinnamoyl-CoA quinate/shikimate hydroxycinnamoyl transferase; CcHQT2 (ACJ23164.1), Cynara cardunculus var. scolymus hydroxycinnamoyl CoA quinate transferase 2; CcHQT1(ADL62854.1), Cynara cardunculus var. scolymus hydroxycinnamoyl-CoA transferase 1; PgHQT(AEM63676.1), Platycodon grandiflorus hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase; LjHQT (ACZ52698), L.japonica HQT; LmHQT1(ALJ56099.1), Lonicera macranthoides HQT1; SlHQT(CAE46933.1), Solanum lycopersicum hydroxycinnamoyl CoA quinate transferase; StHQT (NP_001275483.1), Solanum tuberosum HQT; NtHQT (CAE46932.1), N. tabacum HQT; The TaHQTs protein as labeled after taxon marker. c. Organ expression of TaHQT1 and TaHQT2 gene in wild T.antungese (leave, root, flower, and scape, respectively). Different letters indicate significant differences in comparisons (P <0.05).

Notes

Published as part of Liu, Qun, Yao, Lixiang, Xu, Yachen, Cheng, Haitao, Wang, Weiting, Liu, Zijia, Liu, Jia, Cui, Xin, Zhou, Yujie & Ning, Wei, 2019, In vitro evaluation of hydroxycinnamoyl CoA:quinate hydroxycinnamoyl transferase expression and regulation in Taraxacum antungense in relation to 5-caffeoylquinic acid production, pp. 148-156 in Phytochemistry 162 on page 150, DOI: 10.1016/j.phytochem.2019.02.014, http://zenodo.org/record/10482242

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Journal article: 10.1016/j.phytochem.2019.02.014 (DOI)
Journal article: urn:lsid:plazi.org:pub:FFBAFFA1FFD2FFDAFF86A542FFA2554A (LSID)
Journal article: https://zenodo.org/record/10482242 (URL)