Figure 1 in Integrative microscopy to explore physical and nanomechanical properties of eggshells of diapausing embryos in Rotifera: a proof-of-concept study

Figure 1. Characterisation of diapausing embryos of Brachionus calyciflorus. a. Scanning Electron Microscope image of a single egg. b. Close-up of the surface of the egg. c. Ultrastructure of the shell viewed with Transmission Electron Microscopy. d. Close-up of shell ultrastructure. e. Topographic image of the external surface of an egg generated with Atomic Force Microscopy. f. Force–distance (FD) curve generated from a single location on an egg. Parameters for the position of the location on the eggshell are indicated by the cursor positions (software screenshot). Maximum force (MF, green circle) and 20% maximum force (20% MF, orange circle) are indicated on the FD curve. Abbreviations: al, alveolar layer; ar, amorphous layer around embryo; em, embryo; s1, sublayer. Scale bars: A = 25 µm; B = 5 µm; C = 3.5 µm; D = 1.7 µm.