Published November 30, 2023 | Version v1
Journal article Open

Varieties of spring and winter durum wheat (Triticum durum Desf.) by alleles of the Ppd-A1 gene

Creators

  • 1. Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation

Description

Purpose. Identification and evaluation of the frequencies of dominant and recessive alleles of the Ppd-A1 gene in winter and spring durum wheat varieties of different geographical origins. Methods. DNA isolation, allele-specific PCR, electrophoresis in agarose and polyacrylamide gels and statistical analysis were used in the research. Results. Using diagnostic molecular markers, the genotypes of 81 spring and winter durum wheat varieties from different geographical origins were identified by alleles of the Ppd-A1 gene, which determines differences in photoperiodic sensitivity. Four alleles were found in spring varieties and three in winter varieties (the dominant allele Ppd-A1a.2 was absent). The recessive allele Ppd-A1_del303 was not found in any of the examined varieties. Conclusions. No significant differences were found between winter and spring genotypes in the frequency of one or the other allele. In winter and spring varieties, the recessive allele Ppd-A1_del2ex7 is the most frequent (68.5 and 47.9%, respectively). The recessive allele Ppd-A1b is significantly lower in winter varieties and almost identical in spring varieties. The frequencies of the dominant alleles Ppd-A1a.2 and Ppd-A1a.3 are lower than the two above and generally very low. The Ppd-A1a.2 allele was detected only in the Georgian variety ‘Merliuri’ (spring type); Ppd-A1a.3 – in the Ukrainian varieties ‘Luhanska 7’, ‘Metyska’ (spring) and ‘Koralovyi’ (winter). The possibility of using varieties carrying the dominant alleles Ppd-A1a.2 and Ppd-A1a.3 as donors in hard winter wheat bree­ding programmes is currently being discussed, in order to increase their adaptive potential in conditions of drought and high temperatures and to increase grain yield. The use of marker analysis will ensure the selection of breeding material with the optimal combination of alleles of the Ppd-A1a gene.

Files

292911-Article Text-682512-1-10-20240102.pdf

Files (515.8 kB)

Name Size Download all
md5:2d751738f421e612c6778c426a252108
515.8 kB Preview Download

Additional details

References

  • Fjellheim, S., Boden, S., & Trevaskis, B. (2014). The role of seasonal flowering responses in adaptation of grasses to temperate climates. Frontiers in Plant Science, 5, Article 431. doi: 10.3389/fpls.2014.00431
  • Guedira, M., Xiong, M., Hao, Y. F., Johnson, J., Harrison, S., Marshall, D., & Drown-Guedira, G. (2016). Heading date QTL in winter wheat (Triticum aestivum L.) coincide with major developmental genes VERNALIZATION1 and PHOTOPERIOD1. PloS One, 11(5), Article e0154242. doi: 10.1371/journal.pone.0154242
  • Palamarchuk, A. I. (2015). Especially frost- and winter-resistant on the winter durum wheat. Factors in Experimental Evolution of Organisms, 16, 147–151. [In Ukrainian]
  • Royo, C., Ammar, K., Alfaro, C., Dreisigacker, S., Garcia Del Mo­ral, L. F., & Villegas, D. (2018). Effect of Ppd-1 photoperiod sensiti­vity genes on dry matter production and allocation in durum wheat. Field Crops Research, 221, 358–367. doi: 10.1016/j.fcr.2017.06.005
  • Laurie, D. A. (1997). Comparative genetics of flowering time. Plant Molecular Biology, 35(1/2), 167–177. doi: 10.1023/A:1005726329248
  • Wilhelm, E. P., Turner, A. S., & Laurie, D. A. (2009). Photoperiod insensitive Ppd-A1a mutations in tetraploid wheat (Triticum durum Desf.). Theoretical and Applied Genetics, 118(2), 285–294. doi: 10.1007/s00122-008-0898-9
  • Maccaferri, M., Sanguineti, M. C., Corneti, S., Araus, J. L., Salem, M. B., Bort, J., … Tuberosa, R. (2008). Quantitative trait loci for grain yield and adaptation of durum wheat (Triticum durum Desf.) across a wide range of water availability. Genetics, 178(1), 489–511. doi: 10.1534/genetics.107.077297
  • Bentley, A. R., Turner, A. S., Gosman, N., Leigh, F. J., Maccaferri, M., Dreisigacker, S., … Laurie, D. A. (2011). Frequency of photope­riod-insensitive Ppd-A1a alleles in tetraploid, hexaploid and synthetic hexaploid wheat germplasm. Plant Breeding, 130(1), 10–15. doi: 10.1111/j.1439-0523.2010.01802.x
  • Takenaka, S., & Kawahara, T. (2013). Evolution of tetraploid wheat based on variations in 5′ UTR regions of Ppd-A1: evidence of gene flow between emmer and timopheevi wheat. Genetic Resources and Crop Evolution, 60(7), 2143–2155. doi: 10.1007/s10722-013-9983-2
  • Royo, C., Dreisigacker, S., Alfaro, C., Ammar, K., & Villegas, D. (2016). Effect of Ppd-1 genes on durum wheat flowering time and grain filling duration in a wide range of latitudes. The Journal of Agricultural Science, 154(4), 612–631. doi: 10.1017/S0021859615000507
  • Achilli, A. L., Roncallo, P. F., Larsen, A. O., Dreisigacker, S., & Echenique, V. (2022). Population structure, allelic variation at Rht-B1 and Ppd-A1 loci and its effects on agronomic traits in Argentinian durum wheat. Scientific Reports, 12(1), Article 9629. doi: 10.1038/s41598-022-13563-w
  • Royo, C., Dreisigacker, S., Soriano, J. M., Lopes, M. S., Ammar, K. & Villegas, D. (2020). Allelic variation at the vernalization response (Vrn-1) and photoperiod sensitivity (Ppd-1) genes and their association with the development of durum wheat landraces and modern cultivars. Frontiers in Plant Science, 11, Article 838. doi: 10.3389/fpls.2020.00838
  • Arjona, J. M., Royo, C., Dreisigacker, S., Ammar, K., & Villegas, D. (2018). Effect of Ppd-A1 and Ppd-B1 allelic variants on grain number and thousand kernel weight of durum wheat and their impact on final grain yield. Frontiers in Plant Science, 9, Article 888. doi: 10.3389/fpls.2018.00888
  • Takenaka, S., & Kawahara, T. (2012). Evolution and dispersal of emmer wheat (Triticum sp.) from novel haplotypes of Ppd-1 (photoperiod response) genes and their surrounding DNA sequences. Theoretical and Applied Genetics, 125(5), 999–1014. doi: 10.1007/s00122-012-1890-y
  • Shaw, L. M., Turner, A., Herry, L., Griffiths, S., & Laurie, D. A. (2013). Mutant alleles of Photoperiod-1 in wheat (Triticum aestivum L.) that confer a late flowering phenotype in long days. PLoS One, 8(11), Article e79459. doi: 10.1371/journal.pone.0079459
  • Fait, V., & Balashova, I. (2022). Diversity of Ppd-1 genotypes of spring and winter bread wheat (Triticum aestivum L.) of Ukraine. Visnyk of the Lviv University. Series Biology, 87, 32–44. doi: 10.30970/vlubs.2022.87.03 [In Ukrainian]
  • Worland, A. J., Börner, A., Korzun, V., Li, W. M., Petrovic, S., & Sayers, E. J. (1998). The influence of photoperiod genes on the adaptability of European winter wheats. Euphytica, 100(1/3), 385–394. doi: 10.1023/A:1018327700985
  • Amo, A., Serikbay, D., Song, L., Chen, L., & Hu, Y-G. (2022). Pho­toperiod and vernalization alleles greatly affected phenological and agronomic traits in bread wheat under autumn and spring sowing conditions. Crop and Environment, 1(4), 241–250. doi: 10.1016/j.crope.2022.11.002
  • Lozada, D. N., Carter, A. H., & Mason, R. E. (2021). Unlocking the yield potential of wheat: influence of major growth habit and adaptation genes. Crop Breeding, Genetics and Genomics, 3(2), Article e210004. doi: 10.20900/cbgg20210004
  • Bakuma, A. O., Popovych, Yu. A., Motsnyi, I. I., Chebotar, G. O., & Chebotar, S. V. (2018). Effects of the Ppd-D1a allele on growth rates and agronomical traits in wheat detected by the application of analogous lines. Cytology and Genetics, 52(5), 343–352. doi: 10.3103/S009545271805002X
  • Fayt, V. I, Pogrebnyuk, E. A., Balashova, I. A., & Stelmakh, A. F. (2017). Identification and effects of alleles of Ppd-B1 gene on agronomically valuable traits in recombinant-inbred lines of wheat. Plant Physiology and Genetics, 49(1), 36–46. doi: 10.15407/frg2017.01.036 [In Russian]
  • Palamarchuk, A. I. (2016). Methods and results of winter durum wheat breeding in Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation. Collected Scientific Articles Plant Breeding and Genetics Institute – National Center of Seed and Cultivars Investigation, 27, 54–66. [In Ukrainian]
  • Fait, V. I., & Balashova I. A. (2022). Distribution of photope­riod-insensitive alleles Ppd-D1a, Ppd-B1a and Ppd-B1c in winter common wheat cultivars (Triticum aestivum L.) of various origin. Cytology and Genetics, 56(2), 109–117. doi: 10.3103/S0095452722020049
  • Grogan, S. M., Brown-Guedira, G., Haley, S. D., McMaster, G. S., Reid, S. D., Smith, J., & Byrne, P. F. (2016). Allelic variation in developmental genes and effects on winter wheat heading date in the US Great Plains. PloS One, 11(4), Article e0152852. doi: 10.1371/journal.pone.0152852
  • Whittal, A., Kaviani, M., Graf, R., Humphreys, G., & Navabi, A. (2018). Allelic variation of vernalization and photoperiod response genes in a diverse set of Nortn American high latitude winter wheat genotype. PLoS One, 13(8), Article e0203068. doi: 10.1371/journal.pone.0203068
  • Arjona, J. M., Royo, C., Dreisigacker, S., Ammar, K., Subirà, J., & Villegas, D. (2019). Effect of allele combinations at Ppd-1 loci on durum wheat grain filling at contrasting latitudes. Journal of Agronomy and Crop Science, 206, 64–75. doi: 10.1111/jac.12363

This site uses cookies. Find out more on how we use cookies