Published November 8, 2017
| Version v3.0
Preprint
Open
Comparison of Oxford Nanopore basecalling tools
Creators
- 1. Dept Biochemistry & Molecular Biology and Bio21 Institute, University of Melbourne
Description
This repository uses a bacterial genome to assess the read accuracy and consensus sequence accuracy for Oxford Nanopore Technologies (ONT) basecallers. Albacore v2.1.2 and Scrappie raw v1.1.1 (both developed by ONT) were the best overall performers, and Chiron v0.2 was the best performing third-party basecaller. Consensus sequence accuracies reached approximately 99.5%, revealing that even the best basecallers still have systematic errors. Nanopolish, used with its methylation-aware option, was able to raise consensus accuracy to about 99.9%.
Files
rrwick/Basecalling-comparison-v3.0.zip
Files
(58.6 MB)
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md5:dce2dd844a98efc83325d382e259d612
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Additional details
Related works
- Is supplement to
- https://github.com/rrwick/Basecalling-comparison/tree/v3.0 (URL)