Published December 20, 2023 | Version v1
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Supplemental Movie 3: Simultaneous dual-color imaging of ICC-SM in the colon and adjacent SMCs.

Authors/Creators

  • 1. Dundalk Institute of Technology
  • 2. University of Nevada, Reno School of Medicine
  • 3. University of Nevada Reno

Contributors

  • 1. University of Nevada Reno

Description

Supplemental Movie 3: Simultaneous dual-color imaging of ICC-SM in the colon and adjacent SMCs.   Video shows spontaneous, propagating Ca2+ waves through an ICC-SM network along the submucosal surface of the CM in the proximal colon.  FOVs are from a muscle of a mouse expressing GCaMP6f in ICC (left FOV; colored green) and RCaMP1.07 in SMCs (right FOV; colored red) imaged simultaneously with a 20x objective. The characteristics of the fluorophores are such that there is minimal spectral overlap. Signals in ICC-SM and SMCs are coordinated, showing initiation of each cycle of Ca2+ transients in the ICC-SM network followed by activation of SMCs adjacent to ICC-SM. Bottom panel shows traces from fluorescence images:  ICC-SM transients (green trace) preceded Ca2+ signals in SMCs (red trace). Copied with permission from Reference (133).

 

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Additional details

Identifiers

DOI
10.1152/physrev.00036.2022

Related works

Is part of
Video/Audio: 10.1152/physrev.00036.2022 (DOI)

Funding

National Institute of Diabetes and Digestive and Kidney Diseases
R01 DK120759 R01 DK120759
National Institute of Diabetes and Digestive and Kidney Diseases
R01 DK057236 R01 DK057236
National Institute of Diabetes and Digestive and Kidney Diseases
R01 DK12952 R01 DK12952
National Institute of Diabetes and Digestive and Kidney Diseases
P01 DK 41315 P01 DK 41315

References