Published November 28, 2023
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Four new hypogean species of the genus Triplophysa (Osteichthyes, Cypriniformes, Nemacheilidae) from Guizhou Province, Southwest China, based on molecular and morphological data
Authors/Creators
- 1. Guizhou Normal University, Guiyang, China
- 2. Guiyang Healthcare Vocational University, Guiyang, China
- 3. Zhejiang Forest Resource Monitoring Center, Hangzhou, China|Zhejiang Forestry Survey Planning and Design Company Limited, Hangzhou, China
Description
Recently described cave species of the genus Triplophysa have been discovered in southwestern China, suggesting that the diversity of the genus is severely underestimated and that there may be many undescribed species. In this work, four new species of the genus Triplophysa are described from southwestern Guizhou Province, China, namely Triplophysa cehengensis Luo, Mao, Zhao, Xiao & Zhou, sp. nov. and Triplophysa rongduensis Mao, Zhao, Yu, Xiao & Zhou, sp. nov. from Rongdu Town, Ceheng County, Guizhou, Triplophysa panzhouensis Yu, Luo, Lan, Xiao & Zhou, sp. nov. from Hongguo Town, Panzhou City, Guizhou, and Triplophysa anlongensis Song, Luo, Lan, Zhao, Xiao & Zhou, sp. nov. from Xinglong Town, Anlong County, Guizhou. These four new species can be distinguished from all recognized congeners by a combination of morphological characteristics and significant genetic divergences. The discovery of these species increases the number of known cave species within the genus Triplophysa to 39, making the genus the second most diverse group of cave fishes in China after the golden-line fish genus Sinocyclocheilus. Based on the non-monophyletic relationships of the different watershed systems in the phylogenetic tree, this study also discusses the use of cave species of the genus Triplophysa to determine the possible historical connectivity of river systems.
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References
- Borowsky R (2018) Cavefishes. Current Biology 28(2): R60–R64. https://doi.org/10.1016/j.cub.2017.12.011
- Chen SJ, Peng ZG (2019) Triplophysa sanduensis, a new loach species of nemacheilid (Teleostei: Cypriniformes) from South China. Zootaxa 4560(2): 375–384. https://doi.org/10.11646/zootaxa.4560.2.10
- Chen XY, Yang JX (2005) Triplophysa rosa sp. nov.: A new blind loach from China. Journal of Fish Biology 66(3): 599–608. https://doi.org/10.1111/j.0022-1112.2005.00622.x
- Chen YR, Yang JX, Xu GC (1992) A new blind loach of Triplophysa from Yunnan stone forest with comments on its phylogenetic relationship. Zoological Research 13(1): 17–23. [In Chinese]
- Chen XY, Cui GH, Yang JX (2004) A new cave dwelling fish species of genus Triplophysa (Balitoridae) from Guangxi, China. Zoological Research 25(3): 227–231. [In Chinese]
- Chen S, Sheraliev B, Shu L, Peng Z (2021) Triplophysa wulongensis, a new species of cave-dwelling loach (Teleostei, Nemacheilidae) from Chongqing, Southwest China. ZooKeys 1026: 179–192. https://doi.org/10.3897/zookeys.1026.61570
- Chu XL, Chen YR (1979) A new blind cobitid fish (Pisces, Cypriniformes) from subterranean waters in Yunnan, China. Dong Wu Xue Bao 25(3): 285–287. [In Chinese]
- Chu XL, Chen YR (1990) The fishes of Yunnan, China. Part 2. Science Press, Beijing, 313 pp. [In Chinese]
- Deng T, Wu F, Zhou Z, Su T (2020) Tibetan Plateau: An evolutionary junction for the history of modern biodiversity. Science China. Earth Sciences 63(2): 172–187. https://doi.org/10.1007/s11430-019-9507-5
- Deng SQ, Wang XB, Zhang E (2022) Triplophysa qini, a new stygobitic species of loach (Teleostei: Nemacheilidae) from the upper Chang-Jiang Basin in Chongqing, Southwest China. Ichthyological Exploration of Freshwaters 1178: 1–11. https://doi.org/10.23788/IEF-1178
- Edgar RC (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32(5): 1792–1797. https://doi.org/10.1093/nar/gkh340
- Fricke R, Eschmeyer WN, Van der Laan R (2023) Catalog of fishes: genera, species, references. California Academy of Sciences, San Francisco. http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp [Accessed 25 May 2023]
- George D, Paul M (2010) SPSS for Windows step by step: A Simple study guide and reference, 18.0 Update (11th edn.). Prentice Hall, New Jersey, 408 pp.
- He CL, Zhang E, Song ZB (2012) Triplophysa pseudostenura, a new nemacheiline loach (Cypriniformes: Balitoridae) from the Yalong River of China. Zootaxa 3586(1): 272–280. https://doi.org/10.11646/zootaxa.3586.1.26
- Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh L (2018) UFBoot2: Improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35(2): 518–522. https://doi.org/10.1093/molbev/msx281
- Huang TF, Zhang PL, Huang XL, Wu T, Gong XY, Zhang YX, Peng QZ, Liu ZX (2019) A new cave-dwelling blind loach, Triplophysa erythraea sp. nov. (Cypriniformes: Nemacheilidae), from Hunan Province, China. Zoological Research 40(4): 331–336. https://doi.org/10.24272/j.issn.2095-8137.2019.049
- Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7): 1870–1874. https://doi.org/10.1093/molbev/msw054
- Lan JH, Yang JX, Chen YR (1995) Two new species of the subfamily Nemacheilinae from Guangxi, China (Cypriniformes: Cobitidae). Dong Wu Fen Lei Xue Bao 20(3): 366–372. [In Chinese]
- Lan JH, Gan X, Wu TJ, Yang J (2013) Cave Fishes of Guangxi, China. Science Press, Beijing, 266 pp. [In Chinese]
- Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B (2017) PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34(3): 772–773. https://doi.org/10.1093/molbev/msw260
- Li WX (2004) The three new species of Cobitidae from Yunnan, China. Journal of Jishou University 25(3): 93–96. [Natural Science Edition] [In Chinese]
- Li WX, Zhu ZG (2000) A new species of Triplophysa from cave Yunnan. Journal of Yunnan University 22(5): 396–398. [Natural Science Edition] [In Chinese]
- Li WX, Yanf HF, Chen H, Tao CP, Qi SQ, Han NF (2008) A New Blind Underground Species of the Genus Triplophysa (Balitoridae) from Yunnan, China. Zoological Research 29(6): 674–678. https://doi.org/10.3724/SP.J.1141.2008.06674
- Li J, Lan JH, Chen XY, Du LN (2017a) Description of Triplophysa luochengensis sp. nov.(Teleostei: Nemacheilidae) from a karst cave in Guangxi, China. Journal of Fish Biology 91(4): 1009–1017. https://doi.org/10.1111/jfb.13364
- Li J, Li XH, Lan JH, Du LN (2017b) A new troglobitic loach Triplophysa tianlinensis (Teleostei: Nemacheilidae) from Guangxi, China. Ichthyological Research 64 (3): [1–6]: 295–300. https://doi.org/10.1007/s10228-016-0565-0
- Li CQ, Liu T, Li R (2018) A new species of the genus Plateau loach from caves in Guizhou Province. Journal of Jishou University 39(4): 60–63. https://doi.org/10.13438/j.cnki.jdzk.2018.04.012 [Natural Science Edition] [In Chinese]
- Liu SW, Pan XF, Yang JX, Chen XY (2017) A new cave‐dwelling loach, Triplophysa xichouensis sp. nov. (Teleostei Nemacheilidae) from Yunnan, China. Journal of Fish Biology 90(3): 834–846. https://doi.org/10.1111/jfb.13201
- Liu F, Zeng ZX, Gong Z (2022) Two new hypogean species of Triplophysa (Cypriniformes: Nemacheilidae) from the River Yangtze drainage in Guizhou, China. Journal of Vertebrate Biology 71(22062): 22062. https://doi.org/10.25225/jvb.22062
- Lu ZM, Li XJ, Lü WJ, Huang JQ, Xu TK, Huang G, Qian FQ, Yang P, Chen SG, Mao WN, Zhao YH (2022) Triplophysa xuanweiensis sp. nov., a new blind loach species from a cave in China (Teleostei: Cypriniformes: Nemacheilidae). Zoological Research 43(2): 221–224. https://doi.org/10.24272/j.issn.2095-8137.2021.310
- Ma L, Zhao Y, Yang JX (2019) Cavefish of China. In Encyclopedia of caves (3rd Ed.). Academic Press, Pittsburgh, USA, 237–254. https://doi.org/10.1016/B978-0-12-814124-3.00027-3
- Microsoft Corporation (2016) Microsoft Excel. https://office.microsoft.com/excel
- Ministry of Environmental Protection (2015) Priority areas for biodiversity conservation in China. http://www.mee.gov.cn/gkml/hbb/bgg/201601/t20160105_321061.htm [2015-12-30]
- Min R, Zhao Y, Shi J, Yang J (2022) A new species of Homatula (Teleostei, Cobitoidea, Nemacheilidae) from the Pearl River drainage, Yunnan, China. ZooKeys, 1089: 109–124. https://doi.org/10.3897/zookeys.1089.77203
- Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32(1): 268–274. https://doi.org/10.1093/molbev/msu300
- Parsons S, Jones G (2000) Acoustic identification of twelve species of echolocating bat by discriminant function analysis and artificial neural networks. The Journal of Experimental Biology 203(17): 2641–2656. https://doi.org/10.1242/jeb.203.17.2641
- Polaszek A, Manzari S, Quicke DLJ (2010) Morphological and molecular taxonomic analysis of the Encarsia meritoria species complex (Hymenoptera, Aphelinidae), parasitoids of whiteflies (Hemiptera, Aleyrodidae) of economic importance. Zoologica Scripta 33(5): 403–421. https://doi.org/10.1111/j.0300-3256.2004.00161.x
- Prokofiev AM (2010) Morphological classification of loaches (Nemacheilinae). Journal of Ichthyology 50(10): 827–913. https://doi.org/10.1134/S0032945210100012
- Ran JC, Li WX, Chen HM (2006) A new species of blind loach of Paracobitis from Guangxi, China (Cypriniformes: Cobitidae). Guangxi Shifan Daxue Xuebao. Ziran Kexue Ban 24(3): 81–82. [In Chinese]
- Ren Q, Yang JX, Chen XY (2012) A new species of the genus Triplophysa (Cypriniformes: Nemacheilidae), Triplophysa longliensis sp. nov., from Guizhou, China. Zootaxa 3586(1): 187–194. https://doi.org/10.11646/zootaxa.3586.1.17
- Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Tang L, Zhao YH, Zhang CG (2012) A new blind loach, Oreonectes elongatus sp. nov. (Cypriniformes: Balitoridae) from Guangxi, China. Environmental Biology of Fishes 93(4): 483–449. https://doi.org/10.1007/s10641-011-9943-7
- Vornlocher JR, Lukens WE, Schubert BA, Quan C (2021) Late Oligocene precipitation seasonality in East Asia based on δ13C profiles in fossil wood. Paleoceanography and Paleoclimatology 36(4): e2021PA004229. https://doi.org/10.1029/2021PA004229
- Wang DZ, Li DJ (2001) Two new species of the genus Triplophysa from Guizhou, China (Cypriniformes: Cobitidae). Dong Wu Fen Lei Xue Bao 16(1): 98–101. [In Chinese]
- Wen H, Luo T, Wang Y, Wang S, Liu T, Xiao N, Zhou J (2022) Molecular phylogeny and historical biogeography of the cave fish genus Sinocyclocheilus (Cypriniformes: Cyprinidae) in southwest China. Integrative Zoology 17(2): 311–325. https://doi.org/10.1111/1749-4877.12624
- Wu TJ, Wei ML, Lan JH, Du LN (2018a) Triplophysa anshuiensis, a new species of blind loach from the Xijiang River, China (Teleostei, Nemacheilidae). ZooKeys 744: 67–77. https://doi.org/10.3897/zookeys.744.21742
- Wu WJ, He AY, Yang JX, Du LN (2018b) Description of a new species of Triplophysa (Teleostei: Nemacheilidae) from Guizhou Province, China. Journal of Fish Biology 93(1): 88–94. https://doi.org/10.1111/jfb.13670
- Wu F, Fang X, Yang Y, Dupont-Nivet G, Nie J, Fluteau F, Zhang T, Han W (2022) Reorganization of Asian climate in relation to Tibetan Plateau uplift. Nature Reviews. Earth & Environment 3(10): 684–700. https://doi.org/10.1038/s43017-022-00331-7
- Wu L, Fan C, Lan C, Yu J, Wen H, Yang Q, Xiao N, Zhou J (2023) A long-ignored unique ecosystem of cavefishes in the southern karst: Achievements, challenges, and prospects. Environmental Science and Pollution Research International 30(39): 90489–90499. https://doi.org/10.1007/s11356-023-28806-0
- Xiao W, Zhang Y, Liu H (2001) Molecular systematics of Xenocyprinae (Teleostei: Cyprinidae): taxonomy, biogeography, and coevolution of a special group restricted in East Asia. Molecular Phylogenetics and Evolution 18(2): 163–173. https://doi.org/10.1006/mpev.2000.0879
- Xu W, Che J (2019) From cryptic species to biodiversity conservation in China: Status and prospects. Scientia Sinica Vitae 49(4): 519–530. https://doi.org/10.1360/N052018-00229 [In Chinese]
- Yan YL (2017) The origin and evolution of cave-dwelling group of Triplophysa fishes (Teleostei, Cypriniformes, Nemacheilidae). Master thesis, Southwest University, Chongqing. [In Chinese]
- Yang GY, Yuan FX, Liao YM (1986) A new blind Cobitidae fish from the subterranean water in Xiangxi, China. Huazhong Nongye Daxue Xuebao 5(3): 219–223. [In Chinese]
- Yang JX, Chen XY, Lan JH (2004) Occurrence of two new Plateau indicator loaches of Nemacheilinae (Balitoridae) in Guangxi with Reference to Zoogeographical Significance. Zoological Research 25(2): 111–116. [In Chinese]
- Yang J, Wu TJ, Yang JX (2012) A new cave-dwelling loach, Triplophysa macrocephala (Teleostei: Cypriniformes: Balitoridae), from Guangxi, China. Environmental Biology of Fishes 93(2): 169–175. https://doi.org/10.1007/s10641-011-9901-4
- Yang HF, Li WX, Chen ZM (2016) A new cave species of the genus Triplophysa from Yunnan, China. Zoological Research 37(5): 296–300. https://doi.org/10.13918/j.issn.2095-8137.2016.5.296
- Zhang CG, Shao GZ, Wu HL, Zhao YH (2020) Species Catalogue of China. Vol. 2. Animals, Vertebrates (V), Fishes. Science Press, Beijing. [In Chinese]
- Zhao YH, Zhang CG (2009) Endemic fishes of Sinocyclocheilus (Cypriniformes: Cyprinidae) in China species diversity, cave adaptation, systematics and zoogeography. Science Press, Beijing, 271 pp. [In Chinese]
- Zhao YH, Gozlan RE, Zhang CG (2011) Out of sight out of mind: Current knowledge of Chinese cave fishes. Journal of Fish Biology 79(6): 1545–1562. https://doi.org/10.1111/j.1095-8649.2011.03066.x
- Zheng LP, Du LN, Chen XY, Yang JX (2009) A new species of genus Triplophysa (Nemacheilinae: Balitoridae), Triplophysa longipectoralis sp. nov., from Guangxi, China. Environmental Biology of Fishes 85(3): 221–227. https://doi.org/10.1007/s10641-009-9485-4
- Zheng LP, Yang JX, Chen XY (2012) A new species of Triplophysa (Nemacheilidae: Cypriniformes), from Guangxi, southern China. Journal of Fish Biology 80(4): 831–841. https://doi.org/10.1111/j.1095-8649.2012.03227.x
- Zhu SQ (1989) The loaches of the subfamily Nemacheilinae in China (Cypriniformes: Cobitidae). Jiangsu Science and Technology Publishing House, Nanjing, 150 pp. [In Chinese]
- Zhu SQ, Cao WX (1988) Descriptions of two new species and a new subspecies of Noemacheilinae from Yunnan Province (Cypriniformes: Cobitidae). Dong Wu Fen Lei Xue Bao (1): 95–100. [In Chinese]