Published December 12, 2023
| Version v1
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The potential of graphene oxide surfaces to reduce biofilms formed by uropathogens
Creators
- 1. LEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal
- 2. ALiCE—Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal
- 3. Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
- 4. LSRE-LCM—Laboratory of Separation and Reaction Engineering, Laboratory of Catalysis and Materials, Faculty of Engineering, University of Porto, Porto, Portugal
Description
Polydimethylsiloxane (PDMS), 5 wt.% GNP/PDMS (GNP/PDMS), 1 wt.% graphene oxide (GO)/PDMS (GO1/PDMS), 3 wt.% GO/PDMS (GO3/PDMS), 5 wt.% GO/PDMS (GO5/PDMS)) were analyzed regarding hydrophobicity and roughness. Staphylococcus aureus and Pseudomonas aeruginosa biofilms were developed on these different surfaces under static conditions for 24h at 37°C. The number of biofilm total and culturable cells was quantified by flow cytometry and plate counts, respectively, whereas the biofilm amount was determined by crystal violet staining. Moreover, the mechanisms of action of GO were characterized using the flow cytometer.
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