Control of the phage defense mechanism by Quorum Sensing (QS) in clinical isolates of Klebsiella pneumoniae
Creators
- 1. Grupo de Microbiología Traslacional y Multidisciplinar (MicroTM)-Servicio de Microbiología Instituto de Investigación Biomédica A Coruña (INIBIC); Hospital A Coruña (CHUAC); Universidad de A Coruña (UDC), A Coruña Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA) the behalf of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC)
- 2. Unidad Clínica de Enfermedades Infecciosas y Microbiología Clínica, Hospital Universitario Virgen Macarena, Ins@tuto de Biomedicina de Sevilla (Hospital Universitario Virgen Macarena/CSIC/ Universidad de Sevilla), Sevilla, CIBER de Enfermedades Infecciosas (CIBERINFEC), Ins@tuto de Salud Carlos III, Madrid, Spain; MEPRAM, Proyecto de Medicina de Precisión contra las resistencias An@microbianas
- 3. Laboratorio de Referencia e Inves@gación de Resistencias a An@bió@cos e Infecciones Sanitarias, Centro Nacional de Microbiología, Ins@tuto de Salud Carlos III, Majadahonda, Madrid; CIBER de Enfermedades Infecciosas (CIBERINFEC), Ins@tuto de Salud Carlos III, Madrid, Spain; MEPRAM, Proyecto de Medicina de Precisión contra las resistencias An@microbianas
- 4. Grupo de Microbiología Traslacional y Multidisciplinar (MicroTM)-Servicio de Microbiología Instituto de Investigación Biomédica A Coruña (INIBIC); Hospital A Coruña (CHUAC); Universidad de A Coruña (UDC), A Coruña Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA) the behalf of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) MEPRAM, Proyecto de Medicina de Precisión contra las resistencias Antimicrobianas
Description
Multidrug resistant (MDR) bacteria and the shortage of new antibiotics are a serious health problem and have increased the interest in bacteriophages, with great potential as antimicrobial agents but they can induce resistance. The objective of the present study was to reduce the development of phage resistance in K. pneumoniae strains by inhibiting the Quorum Sensing (QS). The QS inhibition by cinnamaldehyde (CAD) was confirmed indirectly by the reduction of biofilm production and directly by a proteomic analysis. Also, the infection assays showed that the phage resistance mechanisms of the bacteria were inhibited when phage-resistant K. pneumoniae strains were treated with a combination of phages with CAD. Finally, these results were confirmed by proteomic analysis as proteins related to the phage defence such as CBASS (bacterial cyclic oligonucleotide-based anti-phage signalling) and R-M systems as well as tail fiber proteins were present under phage treatment but not with the combination.
Files
Control of the phage defense mechanism by Quorum Sensing (QS) in clinical isolates of Klebsiella pneumoniae.pdf
Files
(9.8 MB)
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Additional details
Dates
- Accepted
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2024-06-26