Published December 4, 2023 | Version v1
Journal article Open

Effects of exposure methods to differences in material analysis of plant tanin in leaves of Psidium guajava l. According to UV-VIS spectroscopic photometry from Tana toraja south Sulawesi

Authors/Creators

Description

Psidium guajava L. leaf is one of the kinds of plants that are traditionally nutritious for treating diseases, previous research stated that Psidium guajava L. has tannin compounds that are known to act as astrigenic, anti diarrhea, antibacterial, and antioxidant. The aim of this study is to determine the effect of the detection method on the difference in the results of the analysis of the levels of tannins in seed leaves (Psidium guajava L.) by UV-Vis spectroscopic photometry and to know the amount of the tannins produced. The extraction methods used were maseration and sochletation. The results showed total tannin levels in the extract using the Maseration method of 3,0875 mg EAT/g sochlettation method at 3,722 mg EAT/g. It can be concluded that the extraction uses a sochletation method that has the highest tanin content compared to the sochleteation method.

Files

PrativiFIN.pdf

Files (421.5 kB)

Name Size Download all
md5:5c306807c0790dbc524cac054f954946
421.5 kB Preview Download

Additional details

References

  • Haryani, T. S., Sari, B. L., & Triastinurmiatiningsih, T. The effectiveness of Padina australis extract as an antibacterial agent for Escherichia coli which causes diarrhea. Phytopharmaca: Pharmaceutical Scientific Journal, 2015. 4(2), 1-9.
  • Oktiarni D, Manaf, Syalfinaf, Suripno. Testing of Guava Leaf Extract (Psidium guajava Linn.) on the Healing of Burn Wounds in Mice (Mus musculus). Universitas Bengkulu; 2015.
  • Andriyani D. Determination of the tannin content of Nephelium lappaceum.L leaves using ultraviolet visible spectrophotometry. Faculty of Pharmacy, Muhammadiyah University of Purwokerto; 2019.
  • Nurhasnawati H, Sukarmi, Handayani F. Comparison of maceration and soxhletation extraction methods on the antioxidant activity of ethanol extract of guava leaves (Syzygium malaccense L.). 2017 May.
  • Ditjen POM. Indonesian Materia Medika, Volume VI. Jakarta: Ministry of Health of the Republic of Indonesia. Page. 103-113 | Amalia NA - Academia.edu. 1995.
  • Pratama M. Analysis of Total Tannin Content of Clove Flower Ethanol Extract (Syzygium aromaticum L.) Using UV-VIS Spectrophotometry Method. JFFI. 2019;6(2):368–73.
  • Alasa AN, Anam S, Jamaluddin J. Analysis of total secondary metabolite levels of ethanol extract of tamoenju leaves (Hibiscuss urattensis L.).Kovalen. 2017 Dec 28;3(3):258.
  • Irianty RS, Venti SR. Effect of ethanol-water solvent ratio on tannin content in soxhletation of gambier leaves (Uncaria gambir Roxb). 2014;13(1):1–7.
  • Vermerris W, Nicholson, R. Phenolic Compound. Netherland: Springer; 2006. .88-90.
  • Harbone. Phytochemical Methods: Modern Ways of Analyzing Plants. Bandung: ITB; 1996.
  • Skoog, D.A, Holler, F.J, Crouch, S.R. Principles of Instrumental Analysis 6th edition. 6th ed. Canada: Corporation; 2007.
  • Depkes RI. General Standard Parameters of Medicinal Plant Extracts. Jakarta: Directorate General of Drug and Food Control; 2000.
  • Handayani V, Ahmad AR, Phytochemical Laboratory, Faculty of Pharmacy, Indonesian Muslim University, Makassar, Sudir M, Phytochemical Laboratory, Faculty of Pharmacy, Indonesian Muslim University, Makassar. Test of Antioxidant Activity of Methanol Extract of Patikala Flowers and Leaves (Etlingera elatior (Jack) R.M.Sm) Using the DPPH Method. Pharm Sci Res. 2014 Aug;1(2):86–93.
  • Mangunwardoyo, W, Ismaini, L, Endang, S.H. Analysis of Bioactive Compounds from Fresh Picung (Pangium edule Reinw) Seed Extract. Biology News. 2008;9(3).
  • Satiyarti B. Rina, Fatimatuzzara,, Yana Y. Use of Guava Leaf Extract (Psidium guajava L.) as Ovicidation of Mas Snails (Pomacea canaliculata L) Biology Education Study Program, Faculty of Tarbiyah and Teacher Training, UIN Raden Intan Lampung. Biology Education Study Program, Faculty of Tarbiyah and Teacher Training, UIN Raden Intan Lampung.; 2019.
  • Sa'adah L. Isolation and identification of tannin compounds from starfruit leaves (Averrhoa bilimbi L.). Maulana Malik Ibrahim State Islamic University (UIN), Malang; 2010.
  • Ryanata Ebry. Determination of Tannin Types and Determination of Tannin Levels from Ripe Banana Peel (Musa paradisiaca L.) Using Spectrophotometry and Permanganometry. University of Surabaya Student Scientific Journal. 2014; 4(1).
  • Risnasari, I. Tannin. Research Report, Faculty of Agriculture, North Sumatra.; 2002.