Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts

1. System Requirements
Software Dependencies and Operating Systems
The version of our systems to run “DESeq2_Calibration.R” is this.
・language R 
・version.string R version 4.3.0 (2023-04-21)
The datasets are available on “GSE233555” in the National Center for Biotechnology Information (NCBI) database.

2. Installation Guide
To install R, follow the instructions on the official website: https://www.r-project.org/

3. Instructions to Run on Data
1. Open the R script (e.g., DESeq2_Calibration.R) in an R environment.
2. Execute the script by clicking Run.

4.Expected Output
* The csv file of number of ribosomes on each transcript.

5.Expected Run Time for Demo
* The code is expected to run for approximately 1 minutes on a "normal" desktop computer.