Dataset related to article "Neutrophils mediate protection in colitis and carcinogenesis by controlling bacterial invasion and IL-22 production by gdT cells"
Creators
- Carnevale, Silvia1
- Ponzetta, Andrea1
- Rigatelli, Anna1
- Carriero, Roberta1
- Puccio, Simone1, 2
- Supino, Domenico1
- Grieco, Giovanna1, 3
- Molisso, Piera1, 3
- Di Ceglie, Irene1
- Scavello, Francesco1
- Perucchini, Chiara1
- Pasqualini, Fabio3
- Recordati, Camilla4
- Tripodo, Claudio5
- Belmonte, Beatrice5
- Mariancini, Andrea1
- Kunderfranco, Paolo1
- Sciumè, Giuseppe6
- Lugli, Enrico1
- Bonavita, Eduardo1, 3
- Magrini, Elena1
- Garlanda, Cecilia1, 3
- Mantovani, Alberto1, 3, 7
- Jaillon, Sebastien1, 3
-
1.
IRCCS Humanitas Research Hospital
- 2. Consiglio nazionale delle ricerche (CNR)
-
3.
Humanitas University
- 4. University of Milan, Mouse & Animal Pathology Laboratory (MAPLab)
-
5.
University of Palermo
-
6.
Sapienza University of Rome
-
7.
Queen Mary University of London
Description
This record contains raw data related to article "Neutrophils mediate protection in colitis and carcinogenesis by controlling bacterial invasion and IL-22 production by gd T cells"
Abstract
Neutrophils are the most abundant leukocytes in human blood and play a primary role in resistance against invading microorganisms and in the acute inflammatory response. However, their role in colitis and colitis-associated colorectal cancer is still under debate. Therefore, this study aims to dissect the role of neutrophils in these pathological contexts by using a rigorous genetic approach. Neutrophil-deficient mice (Csf3r-/- mice) were challenged with classic models of colitis and colitis-associated colorectal cancer and the role of neutrophils was assessed by histological, cellular and molecular analyses coupled with adoptive cell transfer and correlative analyses using human datasets. Csf3r-/- mice showed increased susceptibility to colitis and colitis-associated colorectal cancer compared to control Csf3r+/+ mice and adoptive transfer of neutrophils in Csf3r-/- mice reverted the phenotype. In colitis, Csf3r-/- mice showed increased bacterial invasion and reduced number of healing ulcers in the colon, indicating compromised regenerative capacity of epithelial cells. Neutrophils were essential for T cell polarization and IL-22 production. In patients with ulcerative colitis, the expression of CSF3R was positively correlated with IL-22 and IL-23 expression. Moreover, gene signatures associated with epithelial cell development, proliferation and antimicrobial response were enriched in CSF3Rhigh patients. Our data support a model where neutrophils mediate protection against intestinal inflammation and colitis-associated colorectal cancer by controlling the intestinal microbiota and driving the activation of an IL-22-dependent tissue repair pathway.
Files
Files
(2.3 GB)
| Name | Size | Download all |
|---|---|---|
|
md5:981319bc8cb29feba142bd7129a56b4b
|
30.3 MB | Download |
|
md5:5e22906eda8769ce2bfd74f5af341ecc
|
34.7 MB | Download |
|
md5:022794114a331a07f7ef28f30285c012
|
19.9 MB | Download |
|
md5:4c8d7a891face5eebf58439809a9b099
|
21.0 MB | Download |
|
md5:c30660c01a0c627820084dc9aae0e4bd
|
28.7 MB | Download |
|
md5:77639a94a90c1f40dca3448b7241f8e0
|
31.6 MB | Download |
|
md5:ab3062bef4d2ea57b5e845f81e444da0
|
35.8 MB | Download |
|
md5:25cca1fd4d7a0045a7ed38b602111abc
|
38.8 MB | Download |
|
md5:a1e17e55c04834d0ee476ba80990d95f
|
22.0 MB | Download |
|
md5:bd0fd647e772a50d57557e21260f7969
|
23.4 MB | Download |
|
md5:3110e791dcb1ec228d1094edeb021f17
|
23.4 MB | Download |
|
md5:3f1b346c2f3996cd6ea3f8b6b6ebd78f
|
26.0 MB | Download |
|
md5:c41060700901fee95278f3780c5f5fad
|
15.2 MB | Download |
|
md5:1c00f78e3bf4d98d6a45495bb1b92c89
|
16.8 MB | Download |
|
md5:86f5193cc5c4f6cc297983069dd6e82a
|
26.5 MB | Download |
|
md5:072884ee0b82a2f13097430aacab4d4c
|
29.1 MB | Download |
|
md5:89b906315f48747c328cfc25a1675343
|
36.7 MB | Download |
|
md5:078b60679bc5723e4d3869d7f09938f5
|
41.9 MB | Download |
|
md5:8a9e32582d28871d43c45efd683a8b27
|
40.8 MB | Download |
|
md5:138f7e59bccab9c1b25a960980bc5166
|
44.6 MB | Download |
|
md5:5ef5801346aa50d86f996e359d5c319b
|
50.0 MB | Download |
|
md5:ed793c1124d716f563378b32b70488e9
|
54.9 MB | Download |
|
md5:e717a78d3746e4aff3aebe9d134a4fd6
|
45.1 MB | Download |
|
md5:5b6f0d98b238a27104e802c7c338403f
|
49.1 MB | Download |
|
md5:141ea6221bdf2c956af661bfeb1f7d16
|
21.4 MB | Download |
|
md5:118fb36141c28fb282f6d3e13df5f79e
|
23.5 MB | Download |
|
md5:feffdb3bb61be31381cbde806db1e136
|
86.5 MB | Download |
|
md5:492ea1604e1fb3429614a8e37d2a907c
|
96.3 MB | Download |
|
md5:220deb7bdf5e49407ed1456a6f2067f2
|
46.0 MB | Download |
|
md5:1937e28a67f8e6e9532003a9baeb2345
|
53.6 MB | Download |
|
md5:76b41aefa8cde66a40906ed7bf2da716
|
138.3 MB | Download |
|
md5:a7a3b4f50eebf9a55c6c5b7765b894e7
|
158.8 MB | Download |
|
md5:ba1c4c9885bede90544eb11aa714b909
|
28.5 MB | Download |
|
md5:64090e40047eed0f25769191819b89ee
|
31.7 MB | Download |
|
md5:7a3375851d69a87f67cc1d4db9e1ddea
|
22.3 MB | Download |
|
md5:0aee472532ea452554dbac11e44d76c3
|
25.4 MB | Download |
|
md5:d45bfbf7de0e1fac3f2cfac89eeac216
|
84.0 MB | Download |
|
md5:8e10b35480da5dfea63ae183527a29d7
|
93.2 MB | Download |
|
md5:5eefa9f0b175ce29e74b1e1085b624be
|
5.9 MB | Download |
|
md5:be731adbacc21a94907bccd08ef296dc
|
6.7 MB | Download |
|
md5:b323445c6b672d57f12c2ef78f635f08
|
45.7 MB | Download |
|
md5:d28034e39d6ead9d092994579e166f1b
|
49.8 MB | Download |
|
md5:4347e688bebaf06ffd99ca20fff918d3
|
34.7 MB | Download |
|
md5:14f3d0a8f091242cd7067ed1784d240b
|
38.0 MB | Download |
|
md5:b59dfb446221ccf03b86e72ec9701a24
|
26.6 MB | Download |
|
md5:d5617c6104531abcf4e449b51a0f8bd8
|
29.3 MB | Download |
|
md5:026900e94130c865d42633d428586577
|
13.9 MB | Download |
|
md5:0e4970fc77cc59d3a74acca0477eadbb
|
15.9 MB | Download |
|
md5:34f23bda4fa119b53b71c2ca54388e37
|
16.8 MB | Download |
|
md5:35abc45339909e2333b43ea7cff8e5a6
|
19.2 MB | Download |
|
md5:afd797e06641733fffa8f44d493718a8
|
21.0 MB | Download |
|
md5:021041d40543673f2b4a08d3257dc77c
|
23.8 MB | Download |
|
md5:e035226ee1339e3eb6cf6a6ba14418a2
|
62.3 MB | Download |
|
md5:6fc768f4597ca8ff8f184a4e2ee08765
|
69.2 MB | Download |
|
md5:8c2d4dfae7fbe33b669792c701d1675a
|
37.9 MB | Download |
|
md5:74d4ee2231f6abc50f7515600d42a7de
|
40.6 MB | Download |
|
md5:c1874c62969b488b4948be7741308793
|
17.4 MB | Download |
|
md5:8a7044e2d38da8e166decc0ef6d2b329
|
19.3 MB | Download |