Published October 21, 2023 | Version v1
Journal article Open

Bioinformatic challenge on prostate cancer and urinary microbiome

Creators

  • 1. The Istanbul University, Faculty of Science, Molecular Biology and Genetics Department, 34134, Vezneciler-Istanbul/ TÜRKİYE
  • 2. The Private Capa hospital, Department of surgery and urology, 34134, Istanbul, Turkey Email: info@mustebasevil.com
  • 3. The Istanbul University, Institute of Science, Molecular Biology and Genetics Section, 34134, Vezneciler-Istanbul/ TÜRKİYE
  • 4. Department of molecular biology and genetic, Istanbul University, Istanbul, Turkey. Email: earican@istanbul.edu.tr

Description

The Bioinformatics Resource Center (BRC) program was developed by the National Institute of Allergy and Infectious Diseases (NIAID) to assist researchers in analyzing the increasing amount of genomic sequences and other omics-related data. In this work, whole-genome sequences of prostate cancer and genitourinary diseases (WGS) were examined for genes utilizing the BV-BRC Bioinformatics Resource Center. using the Usegalaxy program to combine the plasma and gut microbiome sequences from prostate cancer patients. Following that, chromosomes, plasmids, and unclassified sequences were subjected to ARG analysis. As a result of comprehensive genomic analysis of all samples, the S2 sequences were of good quality compared to the other sequences. As for virulence factors, intracellular survival is one of the important virulence factors, a common gene of Salmonella, which was represented in the prostate cancer samples but not in the urine microbiome samples.

Abstract (Spanish)

El programa del Centro de Recursos Bioinformáticos (BRC) fue desarrollado por el Instituto Nacional de Alergias y Enfermedades Infecciosas (NIAID) para ayudar a los investigadores a analizar la creciente cantidad de secuencias genómicas y otros datos relacionados con la ómica. En este trabajo, se examinaron secuencias del genoma completo del cáncer de próstata y enfermedades genitourinarias (WGS) en busca de genes utilizando el Centro de recursos de bioinformática BV-BRC. utilizando el programa Usegalaxy para combinar secuencias de microbioma plasmático y intestinal de pacientes con cáncer de próstata. A continuación, los cromosomas, plásmidos y secuencias no clasificadas se sometieron a análisis ARG. Como resultado de un análisis genómico exhaustivo de todas las muestras, las secuencias S2 eran de buena calidad en comparación con las otras secuencias. En cuanto a los factores de virulencia, la supervivencia intracelular es uno de los factores de virulencia importantes, un gen común de Salmonella, que estuvo representado en las muestras de cáncer de próstata, pero no en las muestras de microbioma de orina.

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Additional details

Additional titles

Translated title (Spanish)
Reto bioinformático sobre el cáncer de próstata y el microbioma urinario

Identifiers

ISSN
2610-7996

Dates

Other
2023-09-25
Publicado

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