Published October 20, 2023 | Version v2
Dataset Open

Secondary Graft Loss After Third-party "Off-The-Shelf" Antigen-Specific T cell Infusion

Creators

  • 1. Children's National Hospital
  • 2. St. Jude Children's Research Hospital
  • 3. Children's Hospital Atlanta
  • 4. National Cancer Institute
  • 5. University of Utah Health Huntsman Cancer Institute

Description

Here, we describe a severe adverse event of virus-specific T cell (VST therapy) in an infant with severe combined immunodeficiency who received VSTs for treatment of CMV viremia post-transplant and developed secondary graft rejection at 1-month post-VST infusion associated with the expansion of VST donor-derived T cells. Deposited here are the bulk repertoire, single-cell gene expression and TCR, and flow cytometry data from the study.

bulk_rep.zip contains tables of alpha/beta TCR sequences for post-infusion, VST donor, and VST product samples, and samples stimulated with neoepitope peptides. 

VST_agg_wTCR_SS.rds is an R Data Serialization file containing a Seurat object of the processed 10X single-cell gene expression and TCR profiling for the post-infusion, VST donor, and VST product samples. TCR information is stored in the meta.data slot.

P0230D neoantigen expansions.acs, P0230D pentamers.acs, and P0230D_MLR.acs contain the flow cytometry data for the neoantigen and mixed lymphocyte reaction experiments.

Files

bulk_tcr.zip

Files (1.2 GB)

Name Size Download all
md5:a80e1467975902da6784dcef6400b29c
3.7 MB Preview Download
md5:9aa6714c3c826645b8e6a6b29ecf0aad
888.1 MB Download
md5:48a7b56cd9152a654d91993b308f8dc3
40.1 MB Download
md5:9bd46841a68f185104353b3853de36bd
124.1 MB Download
md5:e22ed4740496b26f56ccd9618ebdb4c2
109.7 MB Download