Published October 10, 2023 | Version v1
Dataset Open

SMRT PacBio Sequel sequencing of 12 Silene herbarium specimens

  • 1. University of Gothenburg
  • 2. Alzahra University
  • 3. Royal Botanic Garden of Edinburgh
  • 4. Natural History Museum, University of Oslo

Description

The folder includes reads sequenced with SMRT PacBio Sequel from twelve Silene herbarium specimens. Both demultiplexed and non demultiplexed reads are included. The demultiplexed reads are in folder called "demultiplexed.zip", in files starting with "Barcodenumber_speciesnamesamplingyear.fastq". The barcode number and sequence can be found below, in the description. The non demultiplexed reads are in file called "ps_248_001.ccsreads.fastq.gz".

#NebNext Barcodes
            #Adapter('Barcode 1 (forward)',
            #        start_sequence=('BC01', 'CGTGAT'),
            #        end_sequence=('BC01_rev', 'ATCACG')),
            #Adapter('Barcode 2 (forward)',
            #        start_sequence=('BC02', 'ACATCG'),
            #        end_sequence=('BC02_rev', 'CGATGT')),
            #Adapter('Barcode 3 (forward)',
            #        start_sequence=('BC03', 'GCCTAA'),
            #        end_sequence=('BC03_rev', 'TTAGGC')),
            #Adapter('Barcode 4 (forward)',
            #        start_sequence=('BC04', 'TGGTCA'),
            #        end_sequence=('BC04_rev', 'TGACCA')),
            #Adapter('Barcode 5 (forward)',
            #        start_sequence=('BC05', 'CACTGT'),
            #        end_sequence=('BC05_rev', 'ACAGTG')),
            #Adapter('Barcode 6 (forward)',
            #        start_sequence=('BC06', 'ATTGGC'),
            #        end_sequence=('BC06_rev', 'GCCAAT')),
            #Adapter('Barcode 7 (forward)',
            #        start_sequence=('BC07', 'GATCTG'),
            #        end_sequence=('BC07_rev', 'CAGATC')),
            #Adapter('Barcode 8 (forward)',
            #        start_sequence=('BC08', 'TCAAGT'),
            #        end_sequence=('BC08_rev', 'ACTTGA')),
            #Adapter('Barcode 9 (forward)',
            #        start_sequence=('BC09', 'CTGATC'),
            #        end_sequence=('BC09_rev', 'GATCAG')),
            #Adapter('Barcode 10 (forward)',
            #        start_sequence=('BC10', 'AAGCTA'),
            #        end_sequence=('BC10_rev', 'TAGCTT')),
            #Adapter('Barcode 11 (forward)',
            #        start_sequence=('BC11', 'GTAGCC'),
            #        end_sequence=('BC11_rev', 'GGCTAC')),
            #Adapter('Barcode 12 (forward)',
            #        start_sequence=('BC12', 'TACAAG'),
            #        end_sequence=('BC12_rev', 'CTTGTA')),]

 

Files

demultiplexed.zip

Files (433.4 MB)

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md5:3f1cc29c3338618e56df8d733d69ed6e
190 Bytes Preview Download
md5:fd43650b606de81b5d9f8e5703ca928d
433.4 MB Download

Additional details

Funding

European Commission
Plant.ID - Molecular Identification of Plants 765000