ENDOPHYTES FROM VIOLET PLANTS GROWING IN UZBEKISTAN AS A SOURCE OF PANCREATIC LIPASE INHIBITING COMPOUNDS

Obesity is one of the globally widespread diseases, accompanied by hypertension, hyperlipidemia, atherosclerosis, and type II diabetes. The most effective therapeutic approach for treating obesity is the inhibition of pancreatic lipase (PL). Eight isolates of endophytic fungi were obtained from Viola odorata growing in the park areas of Tashkent, Uzbekistan. Screening of PL inhibiting activity of secondary metabolites produced by isolates in vitro showed that the isolates expose inhibitory activity varying from 28.8 to 91,5 %. The extract of VО1R isolates from the root of V.odorata and identified as Aspergillus fisheri revealed the highest inhibition (91.5%), and a low IC50 value of 20 µg/ml is comparable with Xenical as standard. Phytochemical analysis showed that the VO1R extract contains alkaloids, terpenoids, tannins, and flavonoids.