Effects of Media Composition and C/N Ratio on Polyunsaturated Fatty Acid Production by Pythium irregulare

Sixteen media combinations based on four levels of glucose and yeast extract ranged from 1,0% to 4,0% and
0,25% to 1,0%, respectively, and corresponding carbon/nitrogen ratio (C/N) ranged from 4 to 64, were
examined. The suitable C/N for Pythium irregulare LX growth ranged from 12 to 24, while that for fungal
lipid accumulation as well as EPA and AA synthesis was 32. The optimal medium for EPA and AA
production was composed of 2,0% glucose and 0,25% yeast extract, with addition of 0,1% KH₂PO4.