Published September 29, 2023 | Version 1.0.0
Dataset Open

18S rRNA V9 taxonomic classifier

Creators

  • 1. University of New Hampshire
  • 2. Northern Gulf Institute and NOAA AOML

Description

18S metabarcoding databases and naive-bayes classifier specific to the V9 region. Built from the PR2 v5.0.0 database using Qiime2 (version 2021.2).

Primers used:

1391f: GTACACACCGCCCGTC

EukBr: TGATCCTTCTGCAGGTTCACCTAC

File Description 

pr2_seqs.qza     |.   Full length PR2 v5.0.0 sequences

pr2_tax.qza        |    Taxa for PR2 v5.0.0 database

refseqs_V9.qza. |   Sequences for 18S V9 (primers 1391f, EukBrr), extracted from PR2 SSU v5.0.0, generated by qiime2-2021.2 (forward compatible)

classifier_V9.qza |  Unweighted (uniform) naive-bayes classifier for 18S V9 extracted from PR2 SSU v5.0.0, generated by qiime2-2021.2 

   

Notes

"This work was supported by award 21392 to the Northern Gulf Institute from NOAA/OAR Ocean Acidification Program."

Files

Files (67.0 MB)

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md5:43d43b0da3bce352ca4377fcef9f9303
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md5:3358a8de4db1c3fc0674cc685369f92f
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Additional details

References

  • Burki, Fabien, Andrew J. Roger, Matthew W. Brown, et Alastair G.B. Simpson. 2020. The New Tree of Eukaryotes. Trends in Ecology and Evolution 35 (1): 43‑55. https://doi.org/10.1016/j.tree.2019.08.008.
  • Bokulich NA, Kaehler BD, Rideout JR, Dillon M, Bolyen E, Knight R, Huttley GA, Caporaso JG. 2018. Optimizing taxonomic classification of marker gene sequences. Microbiome 6(1): 90. doi: https://doi.org/10.1186/s40168-018-0470-z.