Published September 29, 2023
| Version 1.0.1
Journal article
Open
RigiFlex and CYANA/MMMx ensemble modeling of tandem RRMs of SRSF1 in free form and bound to two short RNAs
Authors/Creators
- 1. ETH Zürich, Department of Biology
- 2. ETH Zürich, Department of Chemistry and Applied Biosciences
Description
Primary and processed experimental data as well as MMMx modelling and analysis scripts for a study on RNA binding to the tandem RNA-recongnition motifs of Serine/arginine-rich splicing factor 1 (SRSF1). The set comprises the following data and scripts:
- Primary DEER (EPR) data for the free protein and for complexes with RNA UCAUUGGAU as well as UGGAUUUUUCAU
- Processed DEER data (distance distributions)
- RigiFlex ensemble modelling scripts for the free protein and the two complexes to be run with MMMx
- EnsembleFit (MMMx) scripts, including NMR paramagnetic relaxation enhancement (PRE) restraints
- EnsembleAnalysis scripts (MMMx) for analyzing the incomplete disorder-to-order transitions from free SRSF1 to the two RNA complexes
Files
DEER_free.zip
Files
(828.1 kB)
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Additional details
Funding
- Swiss National Science Foundation
- New types of information from pulsed electron paramagnetic resonance spectroscopy 200020_188467
- Swiss National Science Foundation
- Role of Disordered Regions in RNA-Binding Proteins for Function and Pathology CRSII5_170976