Image data for: Microrheology of the liquid-solid transition during gelation

This data set contains fluorescence microscopy images for the microrheology experiments reported in T. H. Larsen and E. M. Furst, “Microrheology of the liquid-solid transition during gelation,” Phys. Rev. Lett. 100, 146001 (2008). 

File descriptions:

1. pa.tar.gz - data for polyacrylamide gels. Polyacrylamide monomer, bis-acrylamide cross-linker, TEMED catalyst, and ammonium persulfate initiator. Stock solutions are made and degassed prior to use. Samples are prepared at 3.0 wt % total acrylamide, including up to 0.1 wt % bis-acrylamide, 0.5 wt % ammonium persulfate, and 0.1% TEMED. Uncompressed size: 29GB
2. peptide.tar.gz - data for a peptide hydrogelator. The final sample contains 0.15 wt % peptide in pH 8.5 buffer (50 mM BTP). Uncompressed size: 27GB

Fluorescent polystyrene microspheres with diameter 1µm serve as tracer particles in both samples. They have a final concentration of 0.15 v % before gelation is initiated. See Larsen and Furst (2008) for further details.

Each compressed tar file expands into individual folders corresponding to the data time point or composition. The folders contain 800 uncompressed TIFF files (2MB / image). Images are taken at 30 frames per second with a 1 millisecond exposure. The conversion for pixels to micrometers is 40.6 pixels / 10 µm.

This work is marked with CC0 1.0 Universal. To view a copy of this license, visit http://creativecommons.org/publicdomain/zero/1.0