Published September 7, 2023 | Version v1
Journal article Open

FLUTE: a Python GUI for interactive phasor analysis of FLIM data

  • 1. Laboratory for Optics and Biosciences, École Polytechnique, CNRS, Inserm, Institut Polytechnique de Paris, 91128 Palaiseau, France

Description

This repository contains the Fluorescence lifetime imaging microscopy (FLIM) data relative to the following publication 

Gottlieb, D., Asadipour, B., Kostina, P., Ung, T., & Stringari, C. (2023). FLUTE: A Python GUI for interactive phasor analysis of FLIM data. Biological Imaging, 1-22. doi:10.1017/S2633903X23000211

Fluorescein_embyo.tif stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration for the Embryo.tif stack

Embryo.tif  file contains the fluorescence intensity decay of a zebrafish embryo at 3 days post fertilisation. This file needs to be calibrated with Fluorescein_embyo.tif stack

Fluorescein_hMSC.tif stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration for the hMSC-ZOOM, hMSC control, hMSC_rotenone stacks.

hMSC-ZOOM.tif and hMSC control.tif stacks contain the fluorescence intensity decay of mesenchymal stromal cells in control condition. This files need to be calibrated with Fluorescein_hMSC.tif stack

hMSC_rotenone.tif stack contains the fluorescence intensity decay of mesenchymal stromal cells treated with rotenone. This file needs to be calibrated with Fluorescein_hMSC.tif stack

starch SHG-IRF.tif stack contains the measurement of the SHG signal from starch, with a known lifetime of 0ns, used as calibration for ZF-1100_noEF.tif,  ZF-1100_607-70_filter.tif and ZF-1100_550-49 _filter.tif stacks

ZF-1100_noEF.tif contains the fluorescence intensity decay of the zebrafish embryo at 5 days post fertilization acquired without emission. This file needs to be calibrated with starch SHG-IRF.tif stack.

ZF-1100_607-70_filter.tif contains the fluorescence intensity decay of the zebrafish embryo at 5 days post fertilization acquired with an emission filter a 607/70 nm. This file needs to be calibrated with starch SHG-IRF.tif stack

ZF-1100_550-49 _filter.tif contains the fluorescence intensity decay of the zebrafish embryo at 5 days post fertilization acquired with an emission filter of 549/50 nm. This file needs to be calibrated with starch SHG-IRF.tif stack.

All files have been acquired with the following parameters:

  • temporal bin number = 56
  • laser repetition rates = 80 MHz
  • bin width = 0.223ns

Files

Embryo.tif

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