Fig. 5 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo

Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic representation of the N-terminal region of SbDGAT1 genes. Red arrow indicates the position of truncation in SbDGAT1-1. (B) TAG mutant H1246 strain transformed with SbDGAT1 full-length and truncated variants restores the TAG biosynthesis capability compared with control as visualized in TLC. The spots of triacylglycerol (TAG), diacylglycerol (DAG) and free fatty acids (FFA) are separated in TLC plate (C) Total TAG content of yeast H1246 cells expressed with full-length (SbDGAT1-1 and SbDGAT1-2) and truncated variants (SbDGAT1-1(39-515) and SbDGAT1-1(89-515)). Wild type yeast cells (BY4741) were used as positive control. (D) FA profile of TAG products isolated from yeast H1246 strain expressed with full-length and truncated variants of SbDGAT1 genes. Saturated FA abbreviations are as follows: 10:0, Capric acid; 12:0, Lauric acid; 14:0, Myristic acid; 15:0, Pentadecylic acid; 16:0, Palmitic acid; 18:0, Stearic acid. Mono-unsaturated FA abbreviations are as follows: 14:1, Myristoleic acid; 16:1, Palmitoleic acid; 18:1, Oleic acid. The error bars represent the SD of three biological replicates. Asterisks indicates significant differences according to student t-test results where * = p <0.05, ** = p <0.01. H1246 transformed with the empty pYES2 vector served as negative control and wild type yeast (BY4741) served as a positive control. FA, fatty acid; DW, dry weight. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)