An Intravenous Pancreatic Cancer Therapeutic: Characterization of CRISPR/Cas9n-modified Clostridium novyi-Non Toxic Supplementary Information Histology Slides Part 4

H&E and Gram stained whole slide scans for blinded cohorts from publication titled ‘An Intravenous Pancreatic Cancer Therapeutic: Characterization of CRISPR/Cas9n-modified Clostridium novyi-Non Toxic’ published in PLOS One 2023.

Excerpt from methods section of publication detailing acquisition of this data: "Formalin-fixed tissue samples were processed for paraffin embedding by the means of dehydration, clearing and paraffin infiltration (Lynx II Tissue Processor). The paraffin embedded tissue samples were sectioned using a Leica Rotatory Microtome RM2125 RTS at 5um thickness. Designated tissue sections were subsequently de-paraffined and stained with H&E and gram staining as per standard histology protocols (Leica Autostainer XL). Whole slide scanning (WSI) was performed by a Panoramic 250 whole slide scanner at 20x magnification (3D Histech) using a Carl-Zeiss Plan-Apochromat 20x / NA 0.8 objective."

Please note when downloading the image files that the folder containing all of the .data files cannot contain the corresponding .mxrs file. The .mxrs and the folder containing the .data files must have identical names and be at the same file level (as uploaded). Files of this type can be opened by the open source software FIJI or QuPath, among others.

Cohorts have been uploaded with viewers blinded to treatment groups to allow for unbiased review should it be desired, with a cohort key uploaded separately (doi: ##). Files are grouped so that all major organs from a single mouse are in the same compressed folder.