Single-cell and spatial transcriptomics of cardiac neural crest reveal a dual role of vinculin in Tgf beta signaling and cell-extracellular matrix interaction during cardiac outflow tract development

Single-cell and spatial transcriptomics of cardiac neural crest reveal a dual role of vinculin in Tgf beta signaling and cell-extracellular matrix interaction during cardiac outflow tract development.

Single-cell RNA-seq data analysis:

all.cncc.combined.EMBO.mapped.Rdata: public CNCC single-cell RNA-seq data integration

E13.5_CNCC_merged_updated.Rdata: single-cell RNA-seq data of E13.5 CNCC generated in Elly lab

all.seurat.GFP.Rdata: scRNA-seq data with GFP detected

Spatial transcriptomics analysis:

ST_seuratobj_merge: included 4 spatial slice samples merge object

ST_seuratobj_Heart_region_subset: heart region of 4 spatial samples

ST_seuratobj_Outflowtract_region_subset: OFT region of 4 spatial samples