Video S1

Video_S1: Live imaging of the TUBG meshwork (arrowheads and arrows show γ-tubules and centrosomes, respectively) in U2OS cells stably co-expressing TUBG shRNA and a TUBG1 green fluorescence protein shRNA-resistant gene. The TUBG meshwork was monitored with a combination of time-lapse (every 300 seconds during 255 minutes, 51 frames) and Z-stack confocal microscopy. The Z-stack series shows maximum-intensity projections of 10 sequential confocal images that were collected at 1 μm intervals for the capturing of the whole cell.

Video_S2: Red fluorescence-protein-tagged (RFP)-centrin 2 (magenta) labels the centriole of living U2OS cells stably co-expressing TUBG shRNA, a TUBG1 green fluorescence protein shRNA-resistant gene (green) and RFP-Centrin 2 (visualizes the location of endogenous centrioles in a living cell). The Z-stack series show maximum-intensity projections of 10 sequential confocal images that were collected at 1 μm intervals every 150 seconds over a 125-minute (50 frames) period to determine the cellular position of centrosomes and centrioles.

Video_S3: Reduced expression levels of TUBG1 affect centrosome movements. The fluorescence images in the video show RFP-centrin 2-labeled centrioles. The image series shows one lapse of a time-lapse Z-stack (maximum intensity projection of 10 frames per time-point at 1.0 μm intervals) series of RFP-Centrin 2 (to visualize the location of endogenous centrioles in living U2OS-sgTUBG cells) expressing U2OS-sgTUBG cells that coexpressed GFP-Cas9. The centrioles were viewed over a 125-minute (50 frames) period.