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Published July 21, 2023 | Version v1
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Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions

Creators

  • 1. Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118, Kiel, Germany
  • 2. Otto Diels Institute for Organic Chemistry, Kiel University, Otto-Hahn Platz 4, 24098 Kiel, Germany
  • 3. Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Kiel, Kiel University, Am Botanischen Garten 14, 24118, Kiel, Germany and current address is Vienna University of Technology, Karlsplatz 13, 1040 Vienna, Austria
  • 4. Department of Neurosurgery, University Medical Center Kiel, Arnold-Heller-Str. 3, House D, 24105 Kiel, Germany
  • 5. Institute of Clinical Molecular Biology, Kiel University, Rosalind-Franklin-Straße 12, 24105 Kiel, Kiel, Germany
  • 6. Cancer Metabolism Group, Department of Cancer Research, Luxembourg Institute of Health, 6A Rue Nicolas-Ernest Barblé, 1210 Luxembourg, Luxembourg
  • 7. Institute of Clinical Molecular Biology, Kiel University, Rosalind-Franklin-Straße 12, 24105 Kiel, Kiel, Germany and Department of Internal Medicine I, University Medical Center Kiel, Kiel, Germany

Description

This data corresponds to the following paper:

Title: Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions
DOI: 10.1126/sciadv.add3643
Journal: Science Advances
Authors: Josh P. Peters, Arne Brahms, Vivian Janicaud, Maria Anikeeva, Eva Peschke, Frowin Ellermann, Arianna Ferrari, Dana Hellmold, Janka Held-Feindt, Na-mi Kim, Johannes Meiser, Konrad Aden, Rainer Herges, Jan-Bernd Hövener, Andrey N. Pravdivtsev

In the upload the figures 2 - 6 are covered, so all figures from the main manuscript with acquired data.

 

Original abstract for the manuscript:

Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance imaging by more than 10,000 times enabling in vivo metabolic imaging can be performed non-invasively in real-time. Here, we are developing a group of dDNP polarized tracers based on nicotinamide (NAM). We synthesized 1-15N-NAM and 1-15N nicotinic acid and hyperpolarized them with dDNP, reaching (13.0±1.9)% 15N polarization. We found that the lifetime of hyperpolarized 1-15N-NAM is strongly field- and pH-dependent, with T1 being as long as 41 s at a pH of 12 and 1 T while as short as a few seconds at neutral pH and fields below 1 T. The remarkably short 1-15N lifetime at low magnetic fields and neutral pH drove us to establish a unique pH neutralization procedure. Using 15N dDNP and an inexpensive rodent imaging probe designed in-house, we acquired a 15N MRI of 1-15N-NAM (previously hyperpolarized for more than an hour) in less than one second.

Notes

We acknowledge funding from German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept (01ZX1915C), DFG (PR 1868/3-1, HO-4602/2-2, HO-4602/3, GRK2154-2019, EXC2167, FOR5042, TRR287) and financial support by Land Schleswig-Holstein within the funding programme Open Access Publikationsfonds. MOIN CC was founded by a grant from the European Regional Development Fund (ERDF) and the Zukunftsprogramm Wirtschaft of Schleswig-Holstein (Project no. 122-09-053). AP acknowledges Kiel University PMI cluster of excellence for intramural supporting enzymatic LDH activity studies. JM received funding from FNR-INTER (NTER/BMBF/18/13399110). We are also thankful to Nicole Kiweler and Laura Neises from LIH for providing us with K-562 cells.

Files

Raw_data_Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions.zip

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Additional details

Related works

Is supplement to
Journal article: 10.1126/sciadv.add3643 (DOI)