QUANTITATIVE EVALUATION OF CARBOHYDRATE LEVELS IN SEEDS FOR HOME USE BY UV-VISIBLE SPECTROPHOTOMETER

A rapid method was developed for the quantitative estimation of carbohydrates present in the different seeds by UV-VISIBLE SPECTROPHOTOMETER. The sample extract of the almond, cashews, peanuts, walnuts, corn, pistachios,   chickpeas , wheat, barley, soya beans,  were subjected by using anthrone as reagent for the quantitative estimation of the carbohydrate. These samples absorbance was read in uv-visible spectrophotometer at the wavelength of 750nm. The recommendation for the general population is that carbohydrate should supply 50 to 55 percent of total calories, and 130 grams per day (520 calories per day) for male and female adults and for athletes is between 55 and 65 percent of total calories.

Keywords: seeds, uv-visible spectrophotometer, wavelength, anthrone, absorbance.