Published July 4, 2023 | Version v1
Journal article Open

Example FIB-SEM datasets for segmentation, spatial analysis, and visualization of volume electron microscopy datasets

Creators

  • 1. Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital and Faculty of Medicine, Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
  • 2. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA
  • 3. Center for Molecular and Cellular Bioengineering, Technology Platform, Technische Universität Dresden, Dresden, Germang
  • 4. Fondazione Human Technopole, Milano, Italy
  • 5. Helmholtz Imaging, MDC, Berlin, Germany
  • 6. EPFL, Switzerland

Description

This record contains demo FIB-SEM raw data and segmentation masks for the protocol paper:

Müller et al. A modular protocol for segmentation, spatial analysis, and visualization of volume electron microscopy datasets

It contains

- `high_c1_source.tif` raw FIB-SEM stack (16nm^3 resolution) of a mouse pancreatic cell
- `data_golgi` volumetric training data for golgi segmentation
- `data_granules` volumetric training data for secretory granule segmentation
- `high_c1` input folder with segmentation masks for cellsketch
- `mycell.n5` input folder for spatial analysis and visualization

 

Files

data_golgi.zip

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