Published July 15, 2023 | Version Online/Offline
Journal article Open

To Determine the Reliability & Sensitivity of Giemsa Stain by Barr Body Test for Accurate Gender Determination in Buccal Mucosal Cells.

Creators

  • 1. Private Practitioner1
  • 2. Associate Professor2
  • 3. Principal3
  • 4. Private Practitioner4

Description

Sex determination in forensic odontology can be done by either on morphological analysis or molecular analysis. Molecular analysis involves the study of DNA from extracted pulp, cartilage, hair, skin. Buccal mucosa, epithelium attached to denture and toothbrush. Barr bodies, F-bodies, SRY gene, AMEL gene can be studied to determine sex from these samples.

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Additional details

References

  • 1. Kaur N, Sidhu R, Chandra S, Taneja N. Buccal Barr bodies: accuracy and reliability in sex determination. Saudi J Oral Dent Res. 2017;2(7):168-73. 2. Htun KS, Tuu N, Aung YL, Zaw T, Hlaing TM. Gender determination from Barr bodies using Giemsa and Methylene Blue stains in buccal mucosal smears. Proceedings of the 24th Myanmar Military Medical Conference DSOH (500- Beds), Yangon, Myanmar, Feb 2017 Paper No. XXX. 3. Ekanem VJ, Ozoko LEC, Abade PO. Cytological determination of condensed sex chromatin body in buccal smear and peripheral blood film using various nuclear staining techniques. Annals Tropical Pathol. 2012;3(2):133-37. 4. Khorate MM, Dhupar A, Ahmed J, Dinkar AD. Gender determination from pulpal tissue. J Forensic Dent Sci. 2014;6:107-12. 5. Sharma S, David MP, Indira AP. Gender determination from dental pulp. International Journal of Contemporary Medical Research 2017;4(4):821-824.