Genomic epidemiology unveils the dynamics and spatial corridor behind the Yellow Fever virus outbreak in Southern Brazil
Creators
- Marta Giovanetti1
- Francesco Pinotti2
- Camila Zanluca3
- Vagner Fonseca4
- Taishi Nakase5
- Andrea C. Koishi3
- Marcel Tscha3
- Guilherme Soares3
- Gisiane Gruber Dorl3
- Antônio Ernesto M.L Marques3
- Renato Sousa6
- Talita Emile Ribeiro Adelino7
- Joilson Xavier8
- Carla de Oliveira1
- Sandro Patroca9
- Natalia Rocha Guimaraes7
- Hegger Fritsch8
- Maria Angélica Mares-Guia1
- Flavia Levy1
- Pedro Henrique Passos10
- Vinicious Leme da Silva11
- Luiz Augusto Pereira11
- Ana Flávia Mendonça11
- Isabel Luana de Macêdo12
- Davi Emanuel Ribeiro de Sousa12
- Gabriela Rodrigues de Toledo Costa13
- Marcio Botelho de Castro14
- Miguel de Souza Andrade15
- Filipe Vieira Santos de Abreu16
- Fabrício Souza Campos17
- Felipe Campos de Melo Iani7
- Maira Alves Pereira7
- Karina Ribeiro Leite Jardim Cavalcante10
- Andre Ricardo Ribas de Freitas18
- Carlos Frederico Campelo de Albuquerque4
- Eduardo Marques Macário19
- Marlei Pickler Debiasi dos Anjos20
- Rosane Campanher Ramos21
- Aline Alves Scarpellini Campos22
- Adriano Pinter23
- Marcia Chame24
- Livia Abdalla24
- Irina Nastassja Riediger25
- Sérvio Pontes Ribeiro26
- Ana Isabel Bento27
- Tulio de Oliveira28
- Carla Freitas29
- Secretaria de Vigilância em Saúde, SVS, Brazilian Ministry of Health, Brasilia, Federal District, Brazil30
- Allison Fabri1
- Cintia Damasceno Dos Santos Rodrigues1
- Carolina Cardoso Dos Santos1
- Marco Antonio Barreto de Almeida31
- Edmilson dos Santos31
- Jader Cardoso31
- Douglas Adriano Augusto32
- Eduardo Krempser32
- Luís Filipe Mucci33
- Renata Rispoli Gatti34
- Sabrina Fernandes Cardoso35
- João Augusto Brancher Fuck36
- Maria Goretti David Lopes37
- Ivana Lucia Belmonte37
- Gabriela Mayoral Pedroso da Silva37
- Maiane Regina Ferreira Soares37
- Marilia de Melo Santos de Castilhos37
- Joseana Cardoso de Souza e Silva37
- Alceu Bisetto Junior37
- Emanuelle Gemin Pouzato37
- Laurina Setsuko Tanabe37
- Daniele Akemi Arita37
- Ricardo Matsuo37
- Josiane dos Santos Raymundo37
- Paula Cristina Linder Silva37
- Ana Santana Araújo Ferreira Silva37
- Sandra Samila37
- Glauco Carvalho7
- Rodrigo Stabeli4
- Wildo Navegantes4
- Luciano Andrade Moreira38
- Alvaro Gil A. Ferreira38
- Guilherme Garcia Pinheiro39
- Bruno Tardelli Diniz Nunes9
- Daniele Barbosa de Almeida Medeiros9
- Ana Cecília Ribeiro Cruz9
- Rivaldo Venâncio da Cunha40
- Wes Van Voorhis41
- Ana Maria Bispo de Filippis1
- Maria Almiron42
- Edward C. Holmes43
- Daniel Garkauskas Ramos10
- Alessandro Romano10
- José Lourenço44
- Luiz Carlos Junior Alcantara45
- Claudia Nunes Duarte dos Santos3
- 1. Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
- 2. University of Oxford
- 3. Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, Paraná, Brazil
- 4. Organização Pan-Americana da Saúde/Organização Mundial da Saúde, Brasília, Distrito Federal, Brazil
- 5. Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, United Kingdom
- 6. Laboratório de Patologia Veterinária, Hospital Veterinário UFPR, PR Brazil
- 7. Laboratório Central de Saúde Pública do Estado de Minas Gerais, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil
- 8. Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;
- 9. Evandro Chagas Institute, Para, Brazil
- 10. Coordenação Geral das Arboviroses, Secretaria de Vigilância em Saúde/Ministério da Saúde (CGARB/SVS-MS), Brasília, Distrito Federal, Brazil
- 11. Laboratório Central de Saúde Pública Dr. Giovanni Cysneiros, Goiânia,Goiás, Brazil
- 12. Veterinary Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, DF 70636- 200, Brazil
- 13. Environmental Health Surveillance, Directorate of the Federal District (DIVAL), DF 70790-060, Brazil.
- 14. Veterinary Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, DF 70636- 200, Brazil; Graduate Program in Animal Sciences, College of Agronomy and Veterinary Medicine, University of Brasília, Brasília, DF 70910-900, Brazil
- 15. Baculovirus Laboratory, Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasília 70910-900, DF, Brazil
- 16. Insect Behavior Laboratory, Federal Institute of Northern Minas Gerais, Salinas 39560-000, MG, Brazil
- 17. Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil\
- 18. Faculdade de Medicina São Leopoldo Mandic, Campinas, SP, Brazil
- 19. Superintendência de Vigilância em Saúde – SES – Santa Catarina
- 20. Laboratorio central de Saude Publica de Santa Catarina
- 21. Laboratório Central de Saúde Pública do Estado do Rio Grande do Sul
- 22. Secretaria Estadual de Saúde do Rio Grande do Sul, Centro Estadual de Vigilância em Saúde, Porto Alegre, RS, Brasil.
- 23. Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, 05508-000, Brazil
- 24. Oswaldo Cruz Foundation, Biodiversity, Wildlife Health Institutional Platform (PIBSS/Fiocruz), Rio de Janeiro, Brazil
- 25. Laboratório Central de Saúde Pública do Estado do Paraná (Lacen-PR), Curitiba, Paraná, Brazil
- 26. Laboratory of Ecology of Diseases & Forests, NUPEB/ICEB, Federal University of Ouro Preto, Minas Gerais, Brazil
- 27. Pandemic Prevention Initiative, The Rockefeller Foundation, Washington DC, USA
- 28. School for Data Science and Computational Thinking, Faculty of Science and Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa
- 29. Secretaria de Vigilância em Saúde, SVS, Brazilian Ministry of Health, Brasilia, Federal District, Brazil
- 30. Oswaldo Cruz Foundation, Fiocruz, Brasilia, Federal District, Brazil
- 31. Secretaria Estadual de Saúde do Rio Grande do Sul, Centro Estadual de Vigilância em Saúde, Porto Alegre, RS, Brasil
- 32. Plataforma Institucional Biodiversidade e Saúde Silvestre - Centro de Informação em Saúde Silvestre (CISS) - Fiocruz/RJ
- 33. Secretaria da Saúde (São Paulo - Estado). Coordenadoria de Controle de Doenças (CCD). Instituto Pasteur (IP)
- 34. State Department of Health of Santa Catarina, Brazil
- 35. Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil; State Department of Health of Santa Catarina, Brazil
- 36. Diretoria de Vigilância Epidemiológica da Secretaria de Estado da Saúde de Santa Catarina
- 37. Secretaria de Estado da Saúde do Paraná, Brazil
- 38. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Instituto René Rachou—Fiocruz, Belo Horizonte 30190-002, MG, Brazil
- 39. Instituto Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil
- 40. Fundação Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro, Rio de Janeiro, Brazil
- 41. Center for Emerging and Re-emerging Infectious Diseases (CERID), University of Washington
- 42. Pan American Health Organization/World Health Organization, Washington, DC, USA
- 43. Marie Bashir Institute for Infectious Diseases and Biosecurity, School of Life and Environmental Sciences and School of Medical Sciences, University of Sydney, Sydney, NSW, Australia
- 44. BioISI (Biosystems and Integrative Sciences Institute), Faculdade de Ciências da Universidade de Lisboa
- 45. Instituto Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil; Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
Description
Despite the considerable morbidity and mortality of yellow fever virus (YFV) infections in Brazil our understanding of disease outbreaks is hampered by limited viral genomic data. Determining the timing and spatial corridors of YFV spread, as well as the geographic hotspots that link the endemic north of the country with epidemic extra-Amazonian regions, are central to predicting and preventing future outbreak and epidemics. Here, we tracked the recent spread of the virus by integrating genome sequences with both epidemiological and vector data. Through a combination of phylogenetic and epidemiological models we reconstructed the recent transmission history of YFV within different epidemic seasons in Brazil. A suitability index based on the highly domesticated Aedes aegypti was able to capture the seasonality of reported human infections. Spatial modelling revealed spatial hotspots with both past reporting and low vaccination coverage, which coincided with many of the largest urban centres in the Southeast. Phylodynamic analysis unravelled the circulation of three distinct YFV lineages, and provided proof of the directionality of a known spatial corridor of viral spread that connects the endemic North with the extra-Amazonian basin. This study illustrates that genomics linked with eco-epidemiology in a One Health framework can provide new insights into the landscape of YFV transmission, augmenting traditional approaches to infectious disease surveillance and control.
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