Pathological and Immunological Study of Lymph Node Obtained from buffaloes Slaughters in Al-Najaf Abattoir

Abstract

The current study was applied on 200 buffaloes (Bubalus bubalis) slaughtered in AL-Najaf abattoir in order to evaluate the pathological and immunological changes in those buffaloes during the slaughter process. The macroscopic, microscopic and serological studies were done to achieve those Objectives. To evaluate the immunological status of the buffaloes, the IL-1 and TNF-α, levels in the serum were measured. The gross examination of various lymph nodes(mesenteric ,submandibular, parotid and supramammary  lymph node) revealed that 23% of them were have variable lesions. The most common in lymph nodes was color change. The histopathological study revealed that the hyperplastic reaction is the most common change in both lymph nodes 39 %. Other histological changes with variable microscopic changes also seen such as: pigmentation, fibrosis, angiogenesis, congestion, necrosis, fibrosis, ectasia, amyloid deposition, angiogenesis, granuloma and lymphoid tissue depletion also seen . The result of serological study showed there was no significant difference IL-1 and TNF-α except the values of normal and lesions- carrying animal of < 1 year of TNF-α. and more >6 years old of IL- 1.The study was concluded : that there were various macroscopic and microscopic changes in and different lymph nodes at the time of slaughtering, as well as no significant difference between in the levels of IL-1 and TNF-α except with few exceptions .