FLUTE: a Python GUI for interactive phasor analysis of FLIM data

This repository contains the Fluorescence lifetime imaging microscopy (FLIM) data relative to the following publication "FLUTE: a Python GUI for interactive phasor analysis of FLIM data" https://www.biorxiv.org/content/10.1101/2023.03.31.534529v1

Fluorescein_embyo.tif stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration for the Embryo.tif

Embryo.tif  file contains the fluorescence intensity decay of a zebrafish embryo at 3 days post fertilisation.

Fluorescein_embyo.tif stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration for the Embryo.tif stack

Embryo.tif  file contains the fluorescence intensity decay of a zebrafish embryo at 3 days post fertilisation.

Fluorescein_hMSC.tif stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration for the hMSC-ZOOM, hMSC control, hMSC_rotenone stacks.

hMSC-ZOOM.tif and hMSC control.tif stacks contain the fluorescence intensity decay of mesenchymal stromal cells in control condition

hMSC_rotenone.tif stack contains the fluorescence intensity decay of mesenchymal stromal cells treated with rotenone

All files have been acquired with the following parameters:

• temporal bin number = 56
• laser repetition rates = 80 MHz
• bin width = 0.223ns