PHYTOCHEMICAL SCREENING AND ANTI ALZHIEMER ACTIVITY FOR THE PEELS OF CITRUS MAXIMA

The literature survey showed that, only scrappy information was available on this shrub. With this scanty information on this plant consistent expectation of unexplored Phyto chemical profile and pharmacological efficacy under Rutaceae family forms the rationale for the study. Extraction and phytochemical evaluation of them deals with chemical analysis of the extract used for pharmacological screening. Qualitative preliminary phytochemical analysis was performed to detect the phytoconstituents nature and their presence in powder and its various extracts. Ethyl acetate extract showed the presence of steroids, flavanoids, tannins, triterpenoid and phenolic compound. Whereas the ethanolic extract showed the presence of steroids, flavanoids, phenols, phytosterol, gums and mucilage, carbohydrate,   triterpenoids, volatile oil. Aqueous extract showed the presence of steroids, flavanoids, phenols, gums and mucilage, carbohydrate and triterpenoid. Based on the acute toxicity studies, previously reported 1/10^th and 1/5^th (200 and 400 mg/kg) of the maximum tolerated dose (2000 mg/kg B.W) were selected for the in vivo studies. The ethanolic extract of the peels of Citrus maxima was administered orally for fourteen days showed a dose dependent and significant improvement in memory of young mice and it also successfully reversed the memory deficits induced by Scopalamine. Furthermore a significant decreased in cholinergic transmission level in mice brain accounts for its multifarious beneficial effects such as anti alzheimer activity. Ethanolic extract showed the significant anti Alzheimer activity thus it was packed into a column to scrutinize the phytoconstituents present in it.

Key words: Phytochemical, Anti Alzhiemer Activity, Peels Of Citrus Maxima