Dataset Open Access

Molecular architecture of the yeast Mediator complex

Robinson, Philip J; Trnka, Michael J; Pellarin, Riccardo; Greenberg, Charles H; Bushnell, David A; Davis, Ralph; Burlingame, Alma L; Sali, Andrej; Kornberg, Roger D

Data manager(s)
Webb, Benjamin

The 21-subunit Mediator complex transduces regulatory information from enhancers to promoters, and performs an essential role in the initiation of transcription in all eukaryotes. This repository contains files used in the 3-D modeling of the entire Mediator complex, using an integrative modeling approach that combines information from chemical cross-linking and mass spectrometry; X-ray crystallography; homology modeling; and cryo-electron microscopy.

The approach is validated by the use of X-ray crystal structures as internal controls and by consistency with previous results from electron microscopy and yeast two-hybrid screens. The model shows the locations and orientations of all Mediator subunits, as well as subunit interfaces and some secondary structural elements. Segments of 20–40 amino acid residues are placed with an average precision of 20 Å. The model reveals roles of individual subunits in the organization of the complex.

For more information about how to reproduce this modeling, see https://salilab.org/mediator or the README file.

Files (2.9 GB)
Name Size
cluster-all.zip md5:04d5629b78bdd1e726a815696444a651 117.4 MB Download
cluster.0.dcd md5:8ba3bc3b319872b6043ed68187161eea 4.6 MB Download
cluster.1.dcd md5:676dddc6a4ce9cecfc02318f499aa6e5 6.3 MB Download
cluster.2.dcd md5:6b65f4df4c80281c5a902f09ded67d56 1.3 MB Download
cluster.3.dcd md5:e847cae1dc5bbabb2967853fb229e7fe 4.1 MB Download
cluster0.zip md5:4a6cd53ca7d7545de1254d96f0fecb39 35.9 MB Download
cluster1.zip md5:ee5dac35623031dfd5082753b7cc5368 46.5 MB Download
cluster2.zip md5:a8bbbcedac45ceefe7bb3b71335f73bf 12.5 MB Download
cluster3.zip md5:ac9bbc581dab8af36fe48ead33eb5738 32.4 MB Download
clustering_half1.zip md5:1e18fcf0bb09b619e88d58a94e29de7e 236.7 MB Download
clustering_half2.zip md5:8f7a899ce6c947e5239f3b2bee719996 238.2 MB Download
clustering_jackknifing.zip md5:d20a8cf9e4deef581d48ae13804aa702 254.8 MB Download
mediator-v1.0.3.zip md5:3f329d7cd3454496d7d296ce7c544500 48.9 MB Download
run20.tar.xz md5:df844feb37978d158095dcd77c7c3331 263.8 MB Download
run28.tar.xz md5:3f2458015b6cdfb4abc10d4209b57d48 844.6 MB Download
run31.tar.xz md5:6c742c5daaaec48146ed8beeea2eae45 788.0 MB Download

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