Published June 8, 2023
| Version v1
Dataset
Open
Spatial Mapping and Host Linking of Mobile Genetic Elements in Complex Microbiomes - Optimization of single molecule MGE FISH
Creators
- 1. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA
Description
We used Escherichia coli transformed with pJKR-H-tetR plasmids encoding an inducible GFP gene as a model system to assess and optimize MGE-FISH on a confocal microscope. We designed FISH probes for the non-coding strand of the GFP gene, used non-transformed E. coli as a negative control, and tested six different FISH protocols.
Files
Files
(4.7 GB)
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md5:26bf3b7b19f69b9aad2a936bb884481f
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126.9 MB | Download |