Published June 5, 2017 | Version v1
Dataset Open

Methods for Extracting and Characterizing RNA from Urine: for downstream PCR and RNAseq Analysis

  • 1. University of Minnesota Medical School – Duluth 1035 University Drive, Duluth, MN 55812-3031
  • 2. Texas A&M University Medical School, Baylor University Medical Center, Dallas TX USA
  • 3. University of Colorado Anschutz Medical Campus, Aurora, CO 80045
  • 4. University of Minnesota Informatics Institute, University of Minnesota, Minneapolis, Minnesota USA

Description

Readily accessible samples such as urine or blood are seemingly ideal for differentiating and stratifying patients, however, it has proven a daunting task to identify reliable biomarkers in such samples. Noncoding RNA holds great promise as a source of biomarkers distinguishing physiologic wellbeing or illness.

Notes

exRNA, extracellular RNA; tRNA, transfer RNA; tRF, tRNA fragment; rRNA, ribosomal RNA; qRT-PCR, quantitative RT-PCR, miRNA, microRNA; ncRNA, noncoding RNA.

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