METHOD DEVELOPMENT, VALIDATION AND OPTIMIZATION FOR SIMULTANEOUS ESTIMATION OF CELECOXIB AND PIPERINE BY RP-HPLC

It is essential to study the stability of celecoxib in presence of piperine. The stability analysis of the drugs individually (celecoxib/piperine) and in combination (celecoxib with piperine) was carried out at different pH conditions (4.5 and 7.4 pH). Reverse Phase High Performance liquid chromatography method was developed for celecoxib and piperine in combined form. The method was optimized and developed using the design of experiments (DOE). For the optimization, the Design expert trial version 13 was used. The developed method was analyzed using Zodiac C-8 Luna, 5 µm, 100Å, and 150 mm column and methanol: acetonitrile (30:70) with 0.1% of trifluoroacetic acid as mobile phase (1 ml/minute, flow rate) and detected at a wavelength of 342 nm for piperine and 253 nm for celecoxib. Retention time of piperine and celecoxib was found to be 4.751 and 6.685 minutes respectively. The correlation coefficient of both drugs was found to be 0.999. The accuracy of piperine was found to be 99.07-100.61% whereas for celecoxib, it was 99.90-100.31. Overall % RSD was found to be less than 2%. Results showed that the celecoxib stability decreases slightly in the presence of piperine. The method was validated according to the International Conference on Harmonization guidelines and found to be reproducible with peaks showing good resolution with short retention time and can be used for simultaneous estimation of of celecoxib and piperine.