Published October 30, 2022 | Version CC BY-NC-ND 4.0
Journal article Open

Phenotypic and Biochemical Characterization of Rhizobia Associated with Medicago polymorpha Growing in Rajasthan

Creators

  • 1. Microbiology Laboratory, Department of Botany, University of Rajasthan, Jaipur (Rajasthan), India.
  • 2. Assistant professor, Microbiology Laboratory, Department of Botany, University of Rajasthan, Jaipur (Rajasthan), India.

Contributors

Contact person:

  • 1. Assistant professor, Microbiology Laboratory, Department of Botany, University of Rajasthan, Jaipur (Rajasthan), India.

Description

Abstract: In present study, a total of 15 rhizobial isolates were isolated from the root nodules of Medicago polymorpha growing in Jaipur and were characterized for their phenotypic, biochemical and plant growth promoting activities. The root nodules of M. polymorpha were elongated, branched and indeterminate. All isolated rhizobia were highly diverse in their physiological traits. Based on colony morphology, all isolates were categorized into six groups. Major group containing 6 isolates (MP1, MP3, MP9, MP10, MP11 and MP13) showed white, opaque, raised, smooth edges, non-gummy, and mucilaginous characteristics. Four rhizobial strains MP3, MP4, MP9, and MP15 showed salt-tolerance up to 3% and were well adapted to high alkaline conditions and exhibited growth in extremely alkaline media (pH 10). Majority of strains showed positive result for IAA production, nitrate reductase and catalase activity. In addition, citrate utilization, ammonia production, phosphate solubilization and cellulase activity were observed in few isolates. In present study, some rhizobial isolates like MP3, and MP15 were very versatile rhizobia that showed high plant growth promoting activities (IAA production and phosphate solubilization) and were also tolerant to high pH and salt concentration. Such type of superior rhizobia can be part of biofertilizers to enhance legume crop productivity in an ecofriendly manner without application of chemical fertilizers. Our study suggested that rhizobial isolates associated with Medicago polymorpha were significantly diverse in their physiological and biochemical parameters.

Notes

Published By: Lattice Science Publication (LSP) © Copyright: All rights reserved.

Files

B2012102222.pdf

Files (588.9 kB)

Name Size Download all
md5:ba4a1e97bf3a69612d0dfd5e24f00984
588.9 kB Preview Download

Additional details

Related works

Is cited by
Journal article: 2582-9475 (ISSN)

References

  • de Lajudie, P.M., Andrews, M., Ardley, J., Eardly, B., Jumas-Bilak, E., Kuzmanović, N., Lassalle, F., Lindström, K., Mhamdi, R., Martínez-Romero, E. & Moulin, L. (2019). Minimal standards for the description of new genera and species of rhizobia and agrobacteria. International journal of systematic and evolutionary microbiology, 69(7), pp.1852-1863.
  • Gehlot, H.S., Tak, N., Kaushik, M., Mitra, S., Chen, W.M., Poweleit, N., Panwar, D., Poonar, N., Parihar, R., Tak, A., Sankhla, I.S., Ojha, A., Rao, S.R., Simon, M.F., dos Reis Junior, F.B., Perigolo, N., Tripathi, A.K., Sprent, J.I., Young, J.P.W., James, E.K., & Gyaneshwar, P. (2013) An invasive Mimosa in India does not adopt the symbionts of its native relatives. Ann Bot 112:179-196
  • Khuntia, D., Panda, N., Mandal, M., Swain, P., Sahu, S. G., &Pattanayak, S. K. (2022). Symbiotic Effectiveness of Acid Tolerant Nodulating Rhizobia on Growth, Yield and Nutrient Uptake of Pigeon pea (Cajanuscajan L.) in Acidic Alfisols. International Journal of Bio-resource and Stress Management, 13(4):403-410.
  • Sankhla, I.S., Tak, N., Meghwal, R.R., Choudhary, S., Tak, A., Rathi, S., Sprent, J.I., James, E.K., &Gehlot, H.S. (2017). Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India. Plant and soil, 410 (1-2): 21-40.
  • Sankhla I.S., Meghwal, R.R., Choudhary, S., Rathi, S., Tak, N., Tak, A., &Gehlot, H.S. (2018). Molecular characterization of microsymbionts associated with root nodules of Crotalaria burhia Buch.-Ham. ex Benth., a native keystone legume species from Thar Desert of India. Indian Journal of Experimental Biology 56 (06): 373-384.
  • Lopez, Z. C., Friesen, M. L., Von Wettberg, E., New, L., & Porter, S. (2021). Microbial mutualist distribution limits spread of the invasive legume Medicago polymorpha. Biological Invasions, 23(3), 843-856.
  • Charman, N., & Ballard, R. A. (2004). Burr medic (Medicago polymorpha L.) selections for improved N2 fixation with naturalised soil rhizobia. Soil Biology and Biochemistry, 36(8), 1331-1337.
  • Mohamed, S. H., Khalifa, M. O. A., & Babiker, N. N. (2018). Physiological and symbiotic characteristics of rhizobia isolated from medicago polymorpha L and Trigonella stellate L growing in semi-arid regions of Libya. Adv Biosci Bioeng, 6(4), 34-41.
  • Somasegaran, P., & Hoben, H.J. (1994). Handbook for Rhizobia: Methods in Legume- Rhizobium Technology, Springer Verlag, NY.
  • Cappuccino, J.G., & Sherman, N. (2007) Microbiology, A Laboratory Manual (7th ed.). The Benjamin/Cummings Publishing Co., California, USA.
  • Lin, J., Roswanjaya, Y. P., Kohlen, W., Stougaard, J., & Reid, D. (2021). Nitrate restricts nodule organogenesis through inhibition of cytokinin biosynthesis in Lotus japonicus. Nature communications, 12(1), 1-12.
  • Gordon, S. A., & Weber, R. P. (1951). Colorimetric estimation of indoleacetic acid. Plant physiology, 26(1), 192.
  • Adjetey, J. A., & Nxumalo, B. N. G. (2017). Effect of Early Water Deficits on Nodulation and Nitrogen Content of Inoculated Soybean. Bangladesh Agronomy Journal, 20(2), 11-16.
  • Xiao, T. T., Schilderink, S., Moling, S., Deinum, E. E., Kondorosi, E., Franssen, H., Kulikova, O., Niebel, A., & Bisseling, T. (2014). Fate map of Medicago truncatula root nodules. Development, 141(18), 3517-3528.
  • Ren, G. (2018). The evolution of determinate and indeterminate nodules within the Papilionoideae subfamily (Doctoral dissertation, Wageningen University and Research).
  • Djedidi, S., Yokoyama, T., Ohkama-Ohtsu, N., Risal, C. P., Abdelly, C., & Sekimoto, H. (2011). Stress tolerance and symbiotic and phylogenic features of root nodule bacteria associated with Medicago species in different bioclimatic regions of Tunisia. Microbes and environments, 26(1), 36-45.
  • Kang, W., Xu, L., Jiang, Z., & Shi, S. (2019). Genetic diversity and symbiotic efficiency difference of endophytic rhizobia of Medicago sativa. Canadian journal of microbiology, 65(1), 68-83.
  • Sebbane, N., Sahnoune, M., Zakhia, F., Willems, A., Benallaoua, S., & de Lajudie, P. (2006). Phenotypical and genotypical characteristics of root nodulating bacteria isolated from annual Medicago spp. in Soummam Valley (Algeria). Letters in applied microbiology, 42(3), 235-241.
  • Shamseldin, A., & Werner, D. (2005). High salt and high pH tolerance of new isolated Rhizobiumetli strains from Egyptian soils. Current Microbiology, 50(1), 11-16.
  • Nagalingam, S., Nithya, T. V., Gayathri, D., Sagarika, A. S., Supriya, G., Vidya, D., Kumar, B.K., Rasool, A., & Mir, M. I. (2020). Morphological, biochemical and plant growth promoting characterization of rhizobia isolated from root nodule of Cajanus cajan L. Plant Archives, 20(2), 1293-1299.
  • Oryakhil, Q., & Irfan, A. (2020). Morphological biochemical and plant growth promoting characterization of rhizobia isolated from root nodule of Cicer arietinum. Tropical Agroecosystems (TAEC), 1(2), 59-63.
  • Orikasa, Y., Nodasaka, Y., Ohyama, T., Okuyama, H., Ichise, N., Yumoto, I., Morita, N., Wei, M. & Ohwada, T. (2010). Enhancement of the nitrogen fixation efficiency of genetically-engineered Rhizobium with high catalase activity. Journal of bioscience and bioengineering, 110(4), 397-402.
  • Shahzad, F., Shafee, M., Abbas, F., Babar, S., Tariq, M. M., & Ahmad, Z. (2012). Isolation and biochemical characterization of Rhizobium meliloti from root nodules of Alfalfa (Medico sativa). The Journal of Animal and Plant Sciences, 22(2), 522-524.
  • Singh, B., Kaur, R., & Singh, K. (2008). Characterization of Rhizobium strain isolated from the roots of Trigonella foenumgraecum (fenugreek). African Journal of Biotechnology, 7(20)
  • Teresa, M.S., Goma-Tchimbakala, J., Eckzechel, N.S.A., & Aimé, L.A. (2021). Isolation and characterization of native Rhizobium strains nodulating some legumes species in South Brazzaville in Republic of Congo. Advances in Bioscience and Biotechnology, 12(01), 10.
  • Manasa, K., Reddy, R. S., Triveni, S., Kumar, B. K., & Priya, N. G. (2017). Characterization of Rhizobium isolates and their potential PGPR characteristics of different rhizosphere soils of Telangana region, India. Int J Curr Microbiol Appl Sci, 6(5), 2808-13.
  • Singha, B., Mazumder, P. B., & Pandey, P. (2018). Characterization of plant growth promoting Rhizobia from root nodule of two legume species cultivated in Assam, India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 88(3), 1007-1016.
  • Wekesa, C. S., Furch, A. C., & Oelmüller, R. (2021). Isolation and characterization of high-efficiency rhizobia from Western Kenya nodulating with Common bean. Frontiers in microbiology, 12.

Subjects

ISSN: 2582-9475 (Online)
https://portal.issn.org/resource/ISSN/2582-9475
Retrieval Number: 100.1/ijb.B2012102222
https://www.ijb.latticescipub.com/portfolio-item/b2012102222/
Journal Website: www.ijb.latticescipub.com
https://www.ijb.latticescipub.com
Publisher: Lattice Science Publication (LSP)
https://www.latticescipub.com