DETECTION OF FREQUENCY OF BACTERIA CARBAPENEMASE PRODUCING IN PEDIATRIC CASES IN TERTIARY CARE HOSPITAL

Introduction:New strains of bacterial species are now showing multidrug resistance & causing nosocomial and community-acquired infections. The rapid rise in the prevalence and clinical consequences of infection caused by bacteria that produce carbapenemase is a global health concern in pediatric cases.

Material And Method:This study was done in bacteriology laboratory of PCMS & RC, from to February 22 to February 23. Total 268 samples of pediatric patients were included in the study. Identification of bacteria was done by conventional methods. Primary screening of Carbapenemase producing bacteria was done by Kirby-Bauer disk diffusion method by using meropenem (10ug) &Imipenem (10ug) as per as CLSI guideline. Confirmation of Carbapenemase producing bacteria was done by 3 different methods using Boronic acid test, Imipenem-EDTA synergy test & Combination disc test method.

Result:In our study the frequency of carbapenemase producing bacteria were found to be 52 % in pediatric cases. Imipenem-EDTA synergy test & Combination disc test method were found to be more effective in detecting carbapenemase producing bacteria into Boronic acid test.

Conclusions:The present study demonstrates the presence of high level of Multidrug resistant Among carbapenemase producing GNB isolated from pediatric cases as assessed in 2023 in central India.We conclude that the prevalence of phenotypically confirmed carbapenemase producer amongGram negative bacteria is quite useful.The comparison of multiple phenotypic assays for the detection of carbapenemaseproducing bacteria indicates that the Combination disc test and Imipenem-EDTA test provides the highest rate of positivity.