Published October 24, 2022
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FIG. 6 in The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E.-J.Gilbert) Bertault and the importance of taxon-specific PCR primers for identification
Creators
- 1. Department of Ecology and Evolution, University of Lausanne, Biophore building, 1015 Lausanne (Switzerland) ricardo.m.castilho@gmail.com
- 2. Instituto Nacional de Investigação Agrária e Veterinária, I.P., 2780-159 Oeiras (Portugal) anacristina.silva@iniav.pt
- 3. Museum of Natural History and Science of the University of Porto (MHNC-UP) - PO Herbarium, Praça Gomes Teixeira, 4099-002 Porto (Portugal) and Research Center in Biodiversity and Genetic Resources (CIBIO) - Research Network in Biodiversity and Evolutionary Biology (InBIO), University of Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão (Portugal) and BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão (Portugal) and Biology Department, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s/n, 4169-007 Porto (Portugal) cmvvicosa@mhnc.up.pt
- 4. Convevo Portugal Av. António Augusto de Aguiar, N.19 4. Direito - Sala B 1050-012 Lisboa (Portugal) mrcbcastro@gmail.com
- 5. Metyis Management Consulting Alpha Tower, 7 & 8 floor De Entree 69, 1101 BH Amsterdam (The Netherlands) xakazulo@gmail.com
- 6. Mediterranean Institute for Agriculture, Environment and Development (MED), Mitra College, Apartado 94, 7006-554 Évora (Portugal) and Research Center in Biodiversity and Genetic Resources (CIBIO) - Research Network in Biodiversity and Evolutionary Biology (InBIO), University of Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão (Portugal) and BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão (Portugal) oliveira@uevora.pt (corresponding author)
Description
FIG. 6. — Results of PCR amplifications using discriminant probes for Amanita pseudovalens comb. nov., stat. nov.: A, ITS region: ITS3-ApsIr3 (all samples positive with concurring control with primers ITS3-ITS4, not shown); B, LSU region: NLC2R-ApsLr2 (all positive with control NLC2R-LR5); Ode15 and Ode16 were negative and positive, respectively, in another amplification (not shown); M, molecular marker; neg. designates the negative control (water); pos. designates the positive control (the P01 extract).
Notes
Published as part of Arraiano-Castilho, Ricardo, Silva, Ana Cristina, Vila-Viçosa, Carlos, Castro, Mário Rui, Morgado, Luís Neves & Oliveira, Paulo, 2022, The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E.-J.Gilbert) Bertault and the importance of taxon-specific PCR primers for identification, pp. 139-157 in Cryptogamie, Mycologie 20 (6) on page 151, DOI: 10.5252/cryptogamie-mycologie2022v43a6, http://zenodo.org/record/7829510
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